中国兽医科学2024，Vol.54Issue(5)：666-673,8.DOI:10.16656/j.issn.1673-4696.2024.0073
华支睾吸虫排泄/分泌产物对肝纤维化的影响
Effect of excretory/secretory products of Clonorchis sinensis on liver fibrosis
摘要
Abstract
In order to investigate the effect of Clonorchis sinensis excretory/secretory products(ESPs)on rat hepatic stellate cell(HSCs),the HSC-T6 cells were treated with different concentrations of CsESPs,and the effects of CsESPs on cell viability were detected by CCK8 method,and at the same time,MCC950 pretreated cells were used as a control,and RT-qPCR and Western-blot were used to detect the gene and protein expression levels of NLRP3,Caspase-1,IL-1β,α-SMA,Col1α1 and Col3α1 in HSC-T6 cells.The results showed that,compared to the control group,25,50 and 100 μg/mL CsESPs had no significant effect on cell viability.When the concentration of CsESPs reached 50 μg/mL,the relative gene expres-sion levels of NLRP3 and its downstream Caspase-1 and IL-1β were significantly increased compared to the control group,and the mRNA expression levels of α-SMA as the marker gene for HSC-T6 cells activa-tion,and Col1α1 as the fibrosis gene,were also significantly higher compared to the control group.Western-blot results showed that,compared to the control group,50 μg/mL CsESPs significantly in-creased the protein expression levels of NLRP3,Caspase-1,IL-1β,α-SMA,Col1α1 and Col3α1 in HSC-T6 cells.But at MCC950 pretreatment groups,the genes and protein expression levels of these six indica-tors were significantly inhibited.The results indicated that CsESPs can activate HSC-T6 cells and in-duce the activation of NLRP3 inflammasome and collagen expression in cells.The activation of NLRP3 in-flammasome was one of the key links of liver fibrosis caused by Clonorchis sinensis infection.关键词
华支睾吸虫/排泄/分泌产物/肝星状细胞/NLRP3炎性小体/肝纤维化Key words
Clonorchis sinensis/excretory/secretory products/hepatic stellate cells/NLRP3 in-flammasome/liver fibrosis分类
农业科技引用本文复制引用
孙云异,魏伟,毛瑞锋,张馨慧,王佳文,刘雪薇,高俊峰,王春仁..华支睾吸虫排泄/分泌产物对肝纤维化的影响[J].中国兽医科学,2024,54(5):666-673,8.基金项目
国家自然科学基金项目(31972703,32172886) （31972703,32172886）
黑龙江省自然科学基金项目(ZD2022C006) （ZD2022C006）
黑龙江八一农垦大学三横三纵支持计划项目(ZRCQC202204) （ZRCQC202204）
