东方山羊豆营养成分测定及提取物抗氧化活性评价OA北大核心CSTPCD

[Objective]This study was aimed to determine the nutrient components of Galega orientalis and evaluate the antioxidant activity of its extracts,and explore the effects of different extraction solvents on the antioxidant activity of Galega orientalis,so as to provide theoretical reference for developing Galega orientalis-based functional products.[Method]National standards were followed to determine the contents of conventional nutrient components in Galega orientalis.The extract A-E was obtained by extracting Galega orientalis with water and 30％,50％,70％and 90％ethanol solutions,respectively.The contents of total flavonoids,total polyphenols and total polysaccharides in the extracts of Galega orientalis were determined by aluminum trichloride colorimetry,Folin-Ciocalteu and phenol-sulfuric acid methods,respectively.In vitro antioxidant activity of Galega orientalis extracts was evaluated by DPPH and ABTS radical scavenge and total antioxidant capacity assays.Correlation analysis was performed between total flavonoid content,total polyphenol content,total polysaccharide content,DPPH free radical half inhibitory concentration(IC50),ABTS free radical IC50 and ferric ion reducing antioxidant power(FRAP)values of Galega orientalis extracts.[Result]The contents of crude protein,crude fat,crude fiber and ash in Galega orientalis were 19.74％,2.53％,37.10％and 8.79％,respectively.The contents of total flavonoids in extracts B and C were 97.56 and 100.78 mg/g,and the contents of total polyphenols and total polysaccharides in extract C were 89.91 and 38.69 mg/g,respectively,which were significantly higher than those in other extracts(P＜0.05).The DPPH free radical IC50 of extracts C and D were 0.13 and 0.11 mg/mL,and the ABTS free radical IC50 were 0.73 and 0.74 mg/mL,respectively,which were significantly lower than those of other extracts(P＜0.05).The FRAP values of extracts C and D were 1 497.87 and 1 431.85 mmol/g,respectively,which were significantly higher than those of other extracts(P＜0.05).Correlation analysis showed that the contents of total flavonoids and total polyphenols in the extracts of Galega orientalis were extremely significantly negatively correlated with DPPH and ABTS free radical IC50(P＜0.01),and were extremely significantly positively correlated with total antioxidant capacity(P＜0.01).[Conclusion]Galega orientalis was rich in nutrients such as crude protein,crude fat and mineral elements,had high value as animal feed.The contents of total flavonoids and total polyphenols in Galega orientalis extracts had a strong correlation with antioxidant activity.Among them,the content of total flavonoids and total polyphenols in 50％ethanol solution extract was the highest,and the antioxidant activity was the strongest.