基于16S rDNA高通量测序技术分析限制基础饲粮补饲水培大麦苗对籽鹅肠道菌群的影响OA北大核心CSTPCD

[Objective]This experiment was conducted to explore the effects of restricted basal diet supplemented with hydroponic barley seedling on intestinal microflora of Zi geese using 16S rDNA high-throughput sequencing technology.[Method]A total of 160 Zi geese at 28 days of age were randomly divided into 4 groups with 4 replicates in each group and 10 geese per replicate.Geese in group A were fed basal diet ad libitum.Geese in groups B,C and D were fed the basal diet with 85％,72.5％and 60％of the feed intake in group A,respectively,with free access to hydroponic barley seedlings.The experiment lasted for 42 days.Two goose was randomly selected and slaughtered from each replicate at 70 days of age,and the mixed contents of jejunum and ileum and cecum contents were collected as samples for high-throughput sequencing by Illumina MiSeq sequencing platform and bioinformatics analysis.[Result](D In terms of diversity analysis,there were no significant differences in Alpha diversity indexes and results of Beta diversity analysis among all groups(P＞0.05).② Intestinal microflora from 4 groups had 4 common dominant phyla(relative abundanice＞1％):Firmicutes,Proteobacteria,Bacteroidetes and Actinobacteria,7 common dominant genera:Enterococcus,Pseudomonas,Bacteroides,Subdoligranulum,Oscillospira,Faecalibacterium and Helicobacter.Intestinal microflora in group D had significant lower Actinobacteria relative abundance than that of group A(P＜0.05).Intestinal microflora in groups B,C and D had significant lower Spirochaetes,Fusobacteria,Corynebacterium,Staphylococcus and Aerococcus,and significant higher Enterococcus relative abundance than those of group A(P＜0.05).③ LEfSe analysis found that intestinal microflora in group A had 4 labeled species:Actinobacteria,Enhydrobacter,Lactobacillus,Lactobacillaceae.The microflora in group B had 3 labeled species:Chloroplast,Streptophyta and Intrasporangiaceae.The microflora in group C had 11 labeled species:Xanthomonadaceae,Xanthomonadales,Bradyrhizobiaceae,Oxalobacteraceae,Burkholderiales,Ralstonia,beta-Proteobacteria,TM7,TM7_3,Rs_045 and I025.No labeled specie was found in the intestinal microflora in group D.[Conclusion]Under these experimental conditions,restricted basal diet supplemented with hydroponic barley seedling had no significant effect on diversity of intestinal microflora of Zi geese,while could affect relative abundance of microflora,and could inhibit the growth of some pathogenic bacteria and improve intestinal health.However,excessive low proportion of basal diet supply might cause intestinal flora imbalance.