CDC42基因的激活和抑制对烟曲霉极性生长影响的初步探究OACSTPCD
The preliminary study on the effect of activation and inhibition of CDC42 gene on polar growth of Aspergil-lus fumigatus
目的 构建烟曲霉CDC42基因显性激活性(dominant active,DA)和显性抑制性(dominant negative,DN)点突变菌株,初步了解这两种点突变对烟曲霉极性生长以及RAC1基因表达的影响.方法 运用分子生物学方法构建CDC42基因激活性(CDC42G14v)和抑制性(CDC42T19N)菌株;光学显微镜观察点突变菌落特征;RT-PCR方法检测Ku80、DA Cdc42和DN Cdc42菌株中RAC1的表达量.结果 成功构建…查看全部>>
Objective To construct CDC42 gene dominant activation and dominant inhibition point mutant strains and to explore the effects of the two point mutations on the polar growth and RAC1 gene expression of Aspergillus fumigatus.Methods CDC42 gene activation(CDC42G14V)and inhibition(CDC42T19N)strains were constructed by molecular biological methods,the characteristics of point mutation colonies were observed under light microscope,and the expression of RAC1 in Ku8…查看全部>>
张梦楠;于婉婉;马彦
山西医科大学第二医院皮肤性病科,太原 030000山西医科大学第二医院皮肤性病科,太原 030000山西医科大学第二医院皮肤性病科,太原 030000
基础医学
烟曲霉Cdc42点突变极性生长
Aspergillus fumigatusCdc42point mutationpolar growth
《中国真菌学杂志》 2024 (1)
1-7,7
2019年度山西省高等学校优秀成果培育项目(2019KJ025)山西省基础研究计划自然科学研究面上项目(202103021224416)
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