中国畜牧兽医2024，Vol.51Issue(5)：2081-2090,10.DOI:10.16431/j.cnki.1671-7236.2024.05.029
血清4型禽腺病毒感染LMH细胞后干扰素刺激基因在转录水平表达量的动态变化
Dynamic Changes in Expression of Interferon-stimulated Genes at the Transcriptional Level After Infection with Fowl Adenovirus Serotype-4 in LMH cells
摘要
Abstract
[Objective]This study was aimed to investigate the changes of interferon(IFN)and interferon-stimulating genes(ISGs)expression in chicken Leghorn male hepatoma(LMH)infected with Fowl adenovirus serotype-4(FAdV-4),and provide reference basis for the study of the interaction between FAdV-4 and ISGs.[Method]The experiment was conducted by infecting LMH cells with FAdV-4,observing the cellular lesions and collecting cell samples at 0,12,24,36,48,60,72,84 and 96 h post-infection,and the dynamic change patterns of the expression of IFN-αand IFN-β and ISGs genes at the transcriptional level at different time points after infection were detected by Real-time quantitative PCR.[Result]The results showed that after FAdV-4 infection of LMH cells,LMH cells showed typical cytopathic lesions at 48 h.It was detected that FAdV-4 proliferated rapidly at 12 h,and peaked at 60 h.Compared with control group,the expression of IFN-α at the transcriptional level was significantly down-regulated after infection(P＜0.05).Compared with control group,the expression of IFN-β was significantly down-regulated at 12 and 24 h after infection(P＜0.05),and then rapidly and significantly up-regulated to the peak at 36 h(P＜0.05),and remained at a high level at 48 h.After that,the expression declined and stabilized,but was still significantly up-regulated compared with control group(P＜0.05).The expressions of ISG12,IFIT5 and DDIT4 genes did not change much in the early stage of infection,and reached the peak at 96 h after infection(P＜0.05).ZFP313,IFITM3 and Viperin genes did not change much in the early stage of infection,and then rapidly and up-regulated to peak at 36 h(P＜0.05),and remained at a high level at 48 h.After that,the expression declined,but was still significantly up-regulated compared with control group(P＜0.05).CD47,OAS and PKR genes showed down-regulated expression compared with control group.[Conclusion]After FAdV-4 infection of LMH cells,IFN and a variety of ISGs showed regular changes at the transcriptional level,which were associated with the replication of the virus in LMH cells,suggesting that these natural immune factors might play an important role in the anti-FAdV-4 viral response.关键词
血清4型禽腺病毒(FAdV-4)/LMH细胞/干扰素/干扰素刺激基因/转录Key words
Fowl adenovirus serotype-4(FAdV-4)/LMH cells/interferon/interferon-stimulated genes/transcription分类
农业科技引用本文复制引用
万丽军,曾婷婷,黄娇玲,张民秀,谢志勤,李小凤,韦悠,王盛,谢芝勋,任红玉,谢丽基,范晴,罗思思,李孟,张艳芳..血清4型禽腺病毒感染LMH细胞后干扰素刺激基因在转录水平表达量的动态变化[J].中国畜牧兽医,2024,51(5):2081-2090,10.基金项目
广西科技重大专项项目(桂科AA23062050) （桂科AA23062050）
"广西八桂学者"专项(2019A50) （2019A50）
