直接PCR与多重实时PCR检测临床标本的实用性评估OACSTPCD

Objective To evaluate the clinical applicability of direct PCR and DG multiplex real-time PCR in the identifica-tion of dermatophytes strains compared with traditional methods.Methods A total of 184 samples of skin or nail scraps suspected of dermatophytosis were collected and divided into 4 samples.They were examined by direct microscopy,cul-tured,direct PCR and DG multiple real-time PCR,respectively.Using microscopic examination and culture as the gold standard,the efficacy of two skin PCR methods for identifying dermatomycosis was compared.Results The sensitivity of direct PCR and DG-PCR were 64％and 99.2％,respectively.The specificity was 99.6％and 88.1％,respectively.The positive predictive values were 97.5％and 94.7％,respectively.The negative predictive values were 55.9％and 98.1％,re-spectively.Among the 125 positive samples,71 cases(56.8％)were detected by culture,81 cases(64.8％)by direct PCR,and 99 cases(79.2％)by DG-PCR.Conclusion DG-PCR and direct PCR have high practical value in detecting dermatophy-ton infection.