中国畜牧兽医2024，Vol.51Issue(5)：2110-2121,12.DOI:10.16431/j.cnki.1671-7236.2024.05.032
补肾通腑方对脂多糖诱导的认知障碍模型大鼠学习记忆能力的影响及作用机制研究
Study on the Effect and Mechanism of Bushen Tongfu Decoction on the Learning and Memory Ability of LPS-induced Cognitive Impairment Model Rats
摘要
Abstract
[Objective]This study was aimed to investigate the improvement effect and anti-inflammatory protective mechanism of Bushen Tongfu decoction(BTD)on the learning and memory abilities of LPS-induced cognitive impairment model rats.[Method]SPF grade male SD rats of 6-week-old were selected.The rat models of cognitive impairment were established by LPS lateral ventricle injection method.All the successful modeled rats were divided into model group,sodium oligomannate capsules(SOC)group,BTD high-dose,middle-dose and low-dose groups randomly.At the same time,the sham operation control group was set.Drugs were administered to rats by gastric perfusion once a day for 8 weeks.BTD high-dose,middle-dose and low-dose groups were give the drug at 15.12,7.56,3.78 g/kg.SOC group was given the drug at 0.094 g/kg.The model and sham operation control groups were administered with equal volumes of pure water.On the 8th weekend,all the rats were detected the spatial learning and memory ability by Morris water maze.The pathological changes in the hippocampus of rats were detected by hematoxylin eosin(HE)staining.The activation of microglia in the hippocampus was detected by immunofluorescence.The expression levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-1β,N-methyl-D-aspartate receptor(NMDA),γ-aminobutyric acid(GABA)and acetylcholine(Ach)in hippocampus neurons were detected by Western blotting analysis.[Result]Compared with sham operation group,the escape latency and swimming distance of rats in model group were significantly prolonged,the number of across the platform after remove platform were decreased significantly(P＜0.05).The cells were arranged irregularly,sparse,and there were cell vacuoles by the HE staining,the expression of inducible nitric oxide synthase(iNOS)/ionized calcium binding adapter molecule 1(Iba1)were increased and the expression of arginase-1(Arg1)/Iba1 were decreased significantly(P＜0.05),the expression levels of TNF-α,IL-6 and IL-1β were increased significantly and the expression levels of NMDA,GABA and Ach were decreased significantly in hippocampus neurons(P＜0.05).Compared with model group,the escape latency and swimming distance in BDT high-dose,middle-dose,low-dose groups and SOC group were significantly shortened,and the number of times of crossing the original platform after removing the platform was increased significantly(P＜0.05),the cells were arranged tightly and neatly,and the cell morphology tended to be normal by the HE staining,the expression of iNOS/Iba1 were decreased significantly(P＜0.05),the expression of Arg1/Iba1 were decreased significantly only in the BTD high-dose(P＜0.05),the expression levels of TNF-α,IL-6,IL-1βwere decreased significantly and the expression levels of NMDA,GABA,Ach were increased significantly(P＜0.05).[Conclusion]BTD could improve the learning and memory ability of rats with LPS-induced cognitive impairment model.The possible mechanism was inhibiting the activation of proinflammatory microglia and decreasing the levels of TNF-α,IL-6,IL-1β and increasing the levels of NMDA,GABA,and Ach in the hippocampus.关键词
认知障碍/补肾通腑方/中枢神经炎症/脂多糖Key words
cognitive impairment/Bushen Tongfu decoction/neuroinflammation/LPS分类
医药卫生引用本文复制引用
张杰,李思敏,宋晓雨,王旭,许玉珉,段建平,马云枝,赵敏..补肾通腑方对脂多糖诱导的认知障碍模型大鼠学习记忆能力的影响及作用机制研究[J].中国畜牧兽医,2024,51(5):2110-2121,12.基金项目
国家自然科学基金项目(81904264、81904265) （81904264、81904265）
河南省中医药科学研究专项基金(2019ZY2142) （2019ZY2142）
