中国畜牧兽医2024，Vol.51Issue(5)：2219-2227,9.DOI:10.16431/j.cnki.1671-7236.2024.05.042
免疫磁性分离技术在病原微生物检测中的应用
Application of Immunomagnetic Separation Technology in the Detection of Pathogenic Microorganisms
摘要
Abstract
Immunomagnetic separation technology is a pre-treatment technology that separates the target adsorbed on the surface of the magnetic bead from the sample under the magnetic field.After the immune magnetic bead captures the target,the immunemagnetic bead-target complex is magnetically fixed on the tube wall,and the target can be obtained by elution of impurities.The enrichment effect of immunomagnetic separation technology is mainly affected by antibody specificity,magnetic bead diameter,magnetic bead addition amount,incubation time and sample pH,in which antibody specificity is the key to affect the enrichment effect of magnetic beads.Using the characteristics of immunomagnetic beads to adsorb antibodies,the coating step can be replaced in the enzyme-linked immunosorbent assay method,which can greatly reduce the detection time.As a pre-treatment method,immunomagnetic separation technology can improve the sensitivity and efficiency of the detection method when combined with other detection methods.In this review,the technical principle,general separation steps,common influencing factors and the application in enzyme-linked immunosorbent assay,immunochromatography,PCR,Real-time quantitative PCR,loop-mediated isothermal amplification reaction,flow cytometry,electrochemical sensors,liquid chromatography were described,so as to provide reference for the research and application of immunomagnetic separation technology.关键词
免疫磁性分离技术/样本前处理/病原微生物Key words
immunomagnetic separation technology/sample preparation/pathogenic microorganism分类
农业科技引用本文复制引用
张峻豪,胡长敏,匡磊,雷镒妃,赵天睿,许灏钧,陈斌,王丹,韦阳飞,洪灯..免疫磁性分离技术在病原微生物检测中的应用[J].中国畜牧兽医,2024,51(5):2219-2227,9.基金项目
国家重点研发计划项目(2022YFD180070103、2016YFD0500906) （2022YFD180070103、2016YFD0500906）
湖北省重点研发计划项目(2020BBA055) （2020BBA055）
中央高校基本科研业务费专项基金资助(2662020DKPY014) （2662020DKPY014）
