中国畜牧兽医2024，Vol.51Issue(5)：2237-2244,8.DOI:10.16431/j.cnki.1671-7236.2024.05.044
抗菌肽LL-1对肺炎克雷伯菌ATCC 700603的抗菌机制
Antimicrobial Mechanism of Antimicrobial Peptide LL-1 Against Klebsiella pneumoniae ATCC 700603
摘要
Abstract
[Objective]This experiment was aimed to study the antibacterial effect and mechanism of a novel antibacterial peptide LL-1.[Method]The minimum inhibitory concentration(MIC)of antibacterial peptide LL-1 against Klebsiella pneumoniae was detected,and the in vitro antibacterial effect of antibacterial peptide LL-1 against Klebsiella pneumoniae was evaluated through the antibacterial curve.Leakage of alkaline phosphatase(ALP),β-galactosidase,nucleic acid and protein were determined to evaluate the effect of antibacterial peptide LL-1 on the cell membrane and cell wall of Klebsiella pneumoniae.The binding of antibacterial peptide LL-1 to Klebsiella pneumoniae DNA was detected by nucleic acid gel electrophoresis.The effect of antibacterial peptide LL-1 on the morphology of Klebsiella pneumoniae was observed through scanning electron microscopy.[Result]Antibacterial peptide LL-1 had good antibacterial activity against Klebsiella pneumoniae,with a MIC of 50 μg/mL.The antibacterial curve showed that 2 MIC of antibacterial peptide LL-1 could completely inhibit the growth of Klebsiella pneumoniae within 6 h.Antibacterial peptide LL-1 led to the leakage of ALP and β-galactosidase of Klebsiella pneumoniae,and 1/4 MIC of antibacterial peptide LL-1 resulted in a extremely significantly higher leakage rate than the control group(P＜0.01).The detection of nucleic acid and protein leakage further confirmed that antibacterial peptide LL-1 might cause the efflux of intracellular contents in Klebsiella pneumoniae.In addition,antibacterial peptide LL-1 could bind to the DNA of Klebsiella pneumoniae.The results of scanning electron microscope observation suggested that antibacterial peptide LL-1 caused the surface of Klebsiella pneumoniae to become rough,bacterial deformation.[Conclusion]The results showed that antibacterial peptide LL-1 increased the permeability of the cell wall and membrane in Klebsiella pneumoniae,and bound with the DNA of Klebsiella pneumoniae,and thus exerted antibacterial effects.This would lay the foundation for in-depth research on the antibacterial mechanism of antibacterial peptide LL-1 against Klebsiella pneumoniae.关键词
抗菌肽/肺炎克雷伯菌/抗菌机制/细胞壁/细胞膜Key words
antimicrobial peptide/Klebsiella pneumoniae/antimicrobial mechanism/cell wall/cell membrane分类
农业科技引用本文复制引用
连凯琪,王宇航,曾雅静,周玲玲,张元臣,张明亮,张改平,王双山..抗菌肽LL-1对肺炎克雷伯菌ATCC 700603的抗菌机制[J].中国畜牧兽医,2024,51(5):2237-2244,8.基金项目
国家自然科学基金青年项目(31802170) （31802170）
河南省科技攻关计划项目(212102110090) （212102110090）
安阳市重点研发与推广专项(2023C01NY013) （2023C01NY013）
安阳工学院博士后创新实践基地启动经费资助(BHJ2020005) （BHJ2020005）
