植物研究2024，Vol.44Issue(3)：349-360,12.DOI:10.7525/j.issn.1673-5102.2024.03.004
转PsnNAC007高耐旱性小黑杨种质创制及其特性分析
Germplasm Innovation and Characteristic Analysis of Transgenic PsnNAC007 Populus simonii×P.nigra with High Drought Tolerance
摘要
Abstract
Populus simonii×P.nigra is a fast-growing and cold-tolerant tree species with elite wood properties in northeast China.In order to produce a new forest germplasm with more widely planting and improved drought tolerance,this study successfully generated transgenic P.simonii×P.nigra using PsnNAC007,a key drought response transcription factor gene.Growth characteristics,drought tolerant ability,net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),water conductance,cell morphology and wood components of overexpressed PsnNAC007 transgenic plants were analyzed respectively.The results showed that there was no significant difference in growth between transgenic and wild-type plants,but drought survival rate was increased by 26.15%.Under drought condition,transgenic plants exhibited reduced Cleaf and Tr,improved water use efficiency,and lower water conductance loss.Anatomical analysis showed that the overexpression of PsnNAC007 led to more and smaller stem xylem vessels,which were conducive to the continuous and efficient water transport in plants under drought conditions.Based on wood components analysis,lignin deposition in stems of transgenic plants was increased significantly,and monosaccharides that constituted cellulose and hemicellulose showed no significant change.关键词
小黑杨/干旱胁迫/转录因子/遗传转化Key words
Populus simonii×P.nigra/drought stress/transcription factor/genetic transformation分类
农业科技引用本文复制引用
武晓倩,何旭,高境烩,李爽..转PsnNAC007高耐旱性小黑杨种质创制及其特性分析[J].植物研究,2024,44(3):349-360,12.基金项目
国家自然科学基金项目(32001331). （32001331）
