植物研究2024，Vol.44Issue(3)：370-379,10.DOI:10.7525/j.issn.1673-5102.2024.03.006
露珠杜鹃繁育系统及杂交亲和性
Breeding System and Hybridization Affinity of Rhododendron irroratum
摘要
Abstract
To protect germplasm resources and provide technical support for native garden species,the breeding system and hybrid affinity of wild Rhododendron irroratum were studied.The experiments included flowering biological characteristics,the characteristics of breeding system and the cross pollination with fine horticultural varieties of Rhododendron.The process of pollen germination and pollen tube growth were observed by fluorescence microscope,and the fruiting and seed setting were calculated.The results showed that(1)the flowering time of R.irroratum dewdrop was from late April to late May,and pollen viability and stigma receptivity decreased gradually with the extension of flowering time.(2)Hybridization index(OCI)was 3,P/O value was 343.47.(3)The main pollinator was Apis cerana.The breeding system was facultative outbred,self-compatible,requiring pollinators.(4)No apomixis was found in artificial pollination,which could automatically self-pollinate.The fruit setting rates of different strains cross-pollination,strains cross-pollination and self-pollination were 46.96%,45.03%and 37.16%respectively.The average fruit setting rate of artificial pollination was basically consistent with that of natural pollination.(5)The hybrid incompatibility was observed in the parent for artificial cross pollination.Fluorescence microscopy revealed callose embolism in the pollen tube of the mother,which prevented the tube from extending to the ovary.Through observation,it is found that the difficulty of cross compatibility lies in pollen tube growth process.关键词
露珠杜鹃/繁育系统/杂交亲和性/荧光显微观察Key words
Rhododendron irroratum/breeding system/hybridization affinity/fluorescence microscopy分类
农业科技引用本文复制引用
熊文艳,吴雅文,白天,解玮佳,董燕,张敬丽..露珠杜鹃繁育系统及杂交亲和性[J].植物研究,2024,44(3):370-379,10.基金项目
云南科技人才与平台计划(云南省吕英民院士专家工作站:202305AF150155) （云南省吕英民院士专家工作站:202305AF150155）
云南农业联合专项(202301BD070001-139) （202301BD070001-139）
云南农业大学科研启动项目(A2032021013) （A2032021013）
创新引导与科技型企业培育计划(云南省宜良县苗木产业科技特派团:202404BI090014). （云南省宜良县苗木产业科技特派团:202404BI090014）
