紫薇响应盐胁迫和碱胁迫的代谢组分析OA北大核心CSTPCD

To explore the metabolic response mechanism of Lagerstroemia indica'Pink Velour'to salt and alkali stresses,one-year-old asexual cottage seedlings were treated to salt stress(NaCl,pH=7.02)and alkali stress(NaHCO3 and Na2CO3,Na+ mole ratio 2:1,pH=9.52)respectively,and the leaves metabolic changes and differences of the two treatment groups and the control group(CK)were analyzed and compared by using liquid chromatography mass spectrometry(LC-MS).The results showed that 156 and 176 different metabolites were screened in salt and alkali stress group respectively,and 23 different metabolites were screened in both groups,and the rest were unique different metabolites.KEGG enrichment analysis showed that the biosynthesis of secondary metabolites,amino acid metabolism,carbohydrate metabolism,fatty acid metabolism and plant hormone synthesis were main metabolic pathways in response to salt and alkali stress.Glycine,serine and threonine anabolism,ABC transporter protein and Vitamin B6 anabolism were the unique metabolic pathways in salt stress groups.Biosynthesis of valine,leucine,isoleucine,arginine,proline,folic acid and ascorbic acid,and mutual transformation between pentose and glucuronic were the unique metabolic pathway in alkali stress groups.The changes of different metabolites and enriched metabolic pathways might be the main mechanism of L.indica response to the salt stress and alkali stress.