中华临床免疫和变态反应杂志2024，Vol.18Issue(2)：137-147,11.DOI:10.3969∕j.issn.1673-8705.2024.02.006
LncRNA NEAT1通过调节miR-124-3p/KLF4轴改善过敏性鼻炎
Long non-coding RNA NEAT1 modulates allergic rhinitis via regulating miR-124-3p/KLF4 axis
摘要
Abstract
Objective Long non-coding RNA(lncRNA)had been confirmed to play a crucial role in development of allergic rhinitis(AR).The aim of this study is to investigate function and molecular mecha-nism of lncRNA Nuclear Enriched Abundant Transcript 1(NEAT1)in pathogenesis of AR.Methods Ovalbu-min(OVA)-induced AR mouse model and IL-13-induced human nasal epithelial cells(HNECs)model were established.Results NEAT1 was highly expressed in AR mice.Production of inflammatory factors were signifi-cantly inhibited by knock-down of NEAT1 in AR mice and HNECs,as well as reduction of cell apopto-sis.Results of further research demonstrated that up-regulation of miR-124-3p and down-regulation of Krüppel-like factor 4(KLF4)could be reversed by down-regulation of NEAT1,thereby inflammatory responses and cell apoptosis in IL-13-induced HNECs were also regulated.In addition,overexpression of miR-124-3p resulted in significant improvement of viability of HNEC cells,inhibition of cell apoptosis and production of inflammatory factors,as well as expression of KLF4.However,phenomenon mentioned above could be significantly reversed by overexpression of NEAT1.Conclusions High expression of NEAT1 in AR was demonstrated in this study,revealing mechanism of its regulatory function on inflammatory response and cell apoptosis through miR-124-3p/KLF4 signaling pathway.These findings not only provided new perspective for in-depth understanding of molecu-lar mechanism of AR,but also proffered potential targets for further research and treatment of AR.关键词
过敏性鼻炎/LncRNA NEAT1/miR-124-3p/KLF4Key words
allergic rhinitis/LncRNA NEAT1/miR-124-3p/KLF4引用本文复制引用
梁红宇,任宇,刘晓佳,王潇,刘晓玲..LncRNA NEAT1通过调节miR-124-3p/KLF4轴改善过敏性鼻炎[J].中华临床免疫和变态反应杂志,2024,18(2):137-147,11.基金项目
内蒙古自治区科技计划项目(2020GG0082) （2020GG0082）
内蒙古自治区自然科学基金项目(2022QN08030) （2022QN08030）
内蒙古自治区卫生健康委科技计划项目(202201068) Inner Mongolia Autonomous Region Science and Technology Plan Project(2020GG0082) （202201068）
Inner Mongolia Autonomous Region Natural Science Foundation Project(2022QN08030) （2022QN08030）
Inner Mongolia Auton-omous Region Health Commission Science and Technology Plan Project(202201068) （202201068）
