神经药理学报2024，Vol.14Issue(1)：11-17,7.DOI:10.3969/j.issn.2095-1396.2024.01.002
Cyclin D1在黄芪甲苷诱导BMSCs向神经元样细胞分化中的表达
Cyclin D1 Expression During Astragaloside A Induced Neuron-Like Cell Differentiation of BMSCs
摘要
Abstract
Objective:To observe the differentiation of bone marrow mesenchymal stem cells(BMSCs)induced by astragaloside A and expression of Cyclin D1.Methods:BMSCs were induced by astragaloside A with 100 mg·L-1.At l,12,24 h after induetion,the morphological changes of BMSCs were observed with optical microscope and the expressions of Nestin,NSE,Cyclin D1 were identified by immunocytochemical techniques.Cyclin D1 mRNA was detected by RT-PCR.Results:Bone marrow cells were primarily cultured and subcultured.The third passage of BMSCs was purified.After treatment with 100 mg·L-1 astragaloside A,some cells presented processes,were similar to neuron-like cells in morphology,and expressed Nestin and neuron-specific enolase.Nestin expression occurred earlier(P<0.05)and at a higher level(P<0.05)than expression of neuron-specific enolase.At 1 hour after treatment with astragaloside A,cyclin D1 expression was substantially down-regulated in BMSCs at both gene and protein levels.Simultaneously,BMSCs began to express nestin.No significant differences in cyclin D1 expression in BMSCs were detected between 12 and 24 hours post-treatment and the pre-treatment level(P>0.05).Conclusion:Our results indicated that astragaloside A regulated cell cycle,prolonged G1/S phase transition,triggered BMSC differentiation,and finally induced BMSC differentiation into neuronal stem cells.The trophism of astragaloside A accelerated further growth of cell processes and the differentiation of cells into neurons.关键词
黄芪甲苷/BMSCs/分化/神经元样细胞/Cyclin D1Key words
astragaloside A/BMSCs/differentiation/neuron-like cells/Cyclin D1分类
医药卫生引用本文复制引用
王晓寅,王会芳,罗强,张辉,张晓丽..Cyclin D1在黄芪甲苷诱导BMSCs向神经元样细胞分化中的表达[J].神经药理学报,2024,14(1):11-17,7.基金项目
河北省教育厅重点项目(No.ZD2014067) （No.ZD2014067）
