皖产桐桔梗祛痰、平喘、止咳、抗炎有效部位筛选OACSTPCD

Objective:To reveal the phlegm-dispelling,asthma-relieving,cough-relieving,and anti-inflammatory fractions of Platycodonis Radix from Tongcheng city(TPR).Methods:Reflux extraction and macroporous resins were employed to separate different fractions of TPR.The phenol red excretion test of mice was carried out to select the effective fractions of TPR for dispelling phlegm.The mouse model of asthma was induced by histamine phosphate and acetylcholine chloride to select the effective fractions of TPR for relieving asthma.The mouse model of cough was induced by concentrated ammonia solution and used to select the effective fractions of TPR for relieving cough.The mouse model of xylene-induced ear edema was established to select the anti-inflammatory fractions of TPR.Results:The ethanol extract,20%ethanol fraction,and 70%ethanol fraction of TPR all possessed the effects of dispelling phlegm,relieving asthma and cough,and anti-inflammation.Especially,the 70%ethanol fraction showed the strongest effects and exerted the effects in a dose-dependent manner.The 70%ethanol fraction increased the excretion of phenol red in the mouse trachea(P<0.001),prolonged the incubation period of asthma and reduced the mouse number of convulsions and falls induced by histamine phosphate and acetylcholine chloride(P<0.001),and prolonged the incubation period of cough(P<0.001)and mitigated the pathological injuries of the lung tissue that were induced by concentrated ammonia solution.Furthermore,the 70%ethanol fraction reduced the cough frequency and lowered the levels of tumor necrosis factor-alpha(TNF-α),interleukin(IL)-1β,and IL-6 in the serum of mice exposed to concentrated ammonia solution(P<0.001).In addition,the 70%ethanol fraction of TPR reduced the ear edema and lowered the levels of TNF-α,IL-1β,and IL-6 in the serum of mice exposed to xylene(P<0.01,P<0.001).Conclusion:The 70%ethanol elution was the main effective fraction of TPR to dispel phlegm,relieve asthma and cough,and inhibit inflammation.This study provides data support for clarifying the pharmacological basis and mechanism of TPR.