千金子制霜前后提取物对正常及癌性腹水模型小鼠泻下逐水效应与毒性研究OACSTPCD

Objective:To screen the effective fractions of Euphorbiae Semen(ES)and Euphorbiae Semen Pulveratum(ESP)for expelling water and investigate the changes of the water-expelling effect and colonic and renal toxicity before and after powdering.Methods:The mouse model of H22 hepatocellular carcinoma with cancerous ascites was used to screen the effective fraction with ascites fluid volume,fecal water content,and urine volume as indicators.The modeled mice were administrated with the petroleum ether fractions of ES and ESP by gavage,and the water-expelling effects of ES and ESP were evaluated based on the biological effect indicators,organ indices,liver and kidney function indicators,oxidative stress markers,inflammatory mediator levels,and expression of aquaporins(AQPs).Normal mice were used to examine the differences in toxicity of ES before and after powdering based on organ indices,liver and kidney function indicators,oxidative stress markers,and inflammatory mediator levels.Results:The petroleum ether fractions of ES and ESP increased the fecal water content and urine volume and reduced the ascites fluid volume of the modeled mice(P<0.05,P<0.01),while the non-petroleum ether fraction had no obvious effect.Compared with the model group,the administration with the petroleum ether fractions of ES and ESP alleviated the tissue damage,lowered the serum levels of blood urea nitrogen(BUN)and creatine(Cr),increased the superoxide dismutase(SOD)content,reduced the malondialdehyde(MDA)content,inhibited tumor necrosis factor-alpha(TNF-α)and interleukin-1 beta(IL-1β)secretion in the colonic and renal tissue,and down-regulated the protein levels of AQP1 and AQP3 in the colonic and renal tissue.Compared with the blank group,the administration of ES caused different degrees of damage in the colonic and renal tissue,increased secretion of TNF-α and IL-1β and renal function indicators,and significantly lowered the SOD level and elevated the MDA level,which were alleviated in the group administrated with ESP.Conclusion:The petroleum ether fraction is the effective fraction of ES and ESP for expelling water.Powdering attenuated the toxicity while retaining the water-expelling effect of ES.