北华大学学报（自然科学版）2024，Vol.25Issue(4)：460-465,6.DOI:10.11713/j.issn.1009-4822.2024.04.007
多参数流式细胞技术分析髓系白血病细胞相关免疫表型
On Immunophenotypes of Myeloid Leukemia Cells by Using Multiparameter Flow Cytometry
摘要
Abstract
Objective To investigate the immunophenotypes of acute myeloid leukemia(AML)cells and its clinical application value.Method Forty-seven patients with acute myeloid leukemia were enrolled.Multipa-rameter flow cytometry was used to analyze the immunophenotypes of leukemia cells in bone marrow samples,and the correlation between immunophenotyping results,treatment and prognosis was statistically analyzed.Further-more,the clinical application of AML immunophenotyping was explored.Results The positive and highly expre-ssed myeloid antigens in 47 AML patients were CD33(93.62%),CD13(89.36%),MPO(89.36%),CD117(82.98%)and CD64(38.30%).Hematopoietic stem/progenitor cells mainly expressed in CD38(93.62%),CD34(65.96%)and HLA-DR(51.06%).Other antigens were mainly CD56(48.94%),CD7(23.40%)and CD19(19.15%).After the same treatment,23 patients(48.94%)achieved complete remission,and the complete remission rate of patients with positive expression of T cell line antigen CD7 was significantly lower than that of patients with negative expression of CD7(P<0.05).Six patients(26.09%)relapsed within 1 year,and there was no correlation between the expression of any of the antigens(P>0.05).After the follow-up,26 patients(55.32%)died,the mortality of patients with positive expression of NK cell antigen CD56 and T cell antigen CD7 was significantly higher than that of patients with negative expression,and the mortality of patients with positive expression of B cell antigen CD19 was significantly lower than that of patients with negative expression(P<0.05).The mortality rate of patients with immature and mature markers out of synchronous was significantly higher than that of patients with synchronous markers(P<0.05).Conclusion Myeloid antigens CD33,CD13,MPO,CD117 and CD64 are reliable antigen markers in the diagnosis of AML.The immunophenotyping results of AML patients are of great significance for clinicians in determining treatment options and judging prognosis.关键词
多参数流式细胞技术/急性髓系白血病/白血病细胞相关免疫表型/CD分子Key words
multiparameter flow cytometry(MP-FCM)/acute myeloid leukemia(AML)/leukemia-associated immunophenotypes(LAIPs)/CD molecute分类
医药卫生引用本文复制引用
王艺潼,姜佳彤,吴金娜,刘天月,武风云,宋玉国..多参数流式细胞技术分析髓系白血病细胞相关免疫表型[J].北华大学学报（自然科学版）,2024,25(4):460-465,6.基金项目
北华大学研究生创新计划项目(2022019). （2022019）
