大学化学2024，Vol.39Issue(4)：373-381,9.DOI:10.3866/PKU.DXHX202312023
神奇"魔法条"之新冠病毒的快速检测
Rapid Detection of SARS-CoV-2 Using an Innovative"Magic Strip"
摘要
Abstract
Colloidal gold,a marker widely embraced in commercial point-of-care testing(POCT)for immunochromatographic assays,is distinguished by its vivid color characteristics.It is environmentally benign,morphologically stable,and required no further visual modifications.This study details a straightforward process for fabricating test strips that obviate the need for sophisticated,costly equipment.Colloidal gold,synthesized by reducing the chloroauric acid with trisodium citrate,was conjugated to the nucleocapsid N protein antibody of SARS-CoV-2.With the test strip's visible area,a competitive antibody-antigen-antibody sandwich complex forms,with the red test line serving as the basis for qualitative analysis of the virus.This detection approach is visually discernible,user-friendly,rapid(within 10 min),sensitive(detection limit of 0.5 ng∙mL-1)and cost-effective(each strip costing no more than 5 yuan).The experimental process offers students an opportunity to engage with the specific biochemical reactions triggered by antigen-antibody interactions,fostering a deep interest in exploring rapid diagnostic technologies that integrate chemistry,biology,life sciences and nanomaterials.关键词
胶体金/POCT/免疫层析/新冠病毒Key words
Colloidal gold/Point-of-care testing(POCT)/Immune chromatography/SARS-CoV-2 virus分类
社会科学引用本文复制引用
杨迪,魏嘉怡,翟红,王昕,孙太明,宋浩乐,王海雁..神奇"魔法条"之新冠病毒的快速检测[J].大学化学,2024,39(4):373-381,9.基金项目
山西省留学人员科研资助项目(2021-144) （2021-144）
