基础医学与临床2024，Vol.44Issue(6)：772-778,7.DOI:10.16352/j.issn.1001-6325.2024.06.0772
骨髓间充质干细胞缓解模拟微重力对小鼠大脑皮质的不利影响
Bone marrow-derived mesenchymal stem cells relieve the adverse effects of simulated microgravity on mouse cerebral cortex
摘要
Abstract
Objective To explore the protective effects and the underlying mechanisms of bone marrow mesenchymal stem cells(BMSCs)on the brain in microgravity environment.Methods The physiological effects of microgravity were simulated with the hindlimb unloading(HU)mouse model.The animals were divided into 3 groups:the control group(wild-type mice),the hindlimb unloading(HU)group,and the BMSCs treatment group,with six in each.RT-qPCR was adopted to determine the expression levels of pro-inflammatory cytokines(Il-6,Il-1β and Tnf-α)in the cerebral cortex;immunohistochemistry was performed to evaluate the proportions of microglia(IBA-positive)and astrocytes(GFAP-positive);Western blot was used to measure the expression levels of apoptosis-and senescence-related molecules(BAX,BCL-2,p21,and p53).Results Compared with wild-type mice,the expression levels of Il-6,Il-1β and Tnf-α in the cerebral cortex of HU mice were significantly increased(P＜0.05),the expression level of BAX,p21 and p53 was also significantly increased(P＜0.05).However,the expression level of BCL-2 protein were significantly decreased(P＜0.05).The proportion of micro-glia(IBA1 positive)and astrocytes(GFAP positive)was increased(P＜0.05);After BMSCs treatment,the expression level of Il-1β in the cerebral cortex of HU mice was significantly decreased(P＜0.05),the expression levels of BAX,p21 and p53 were significantly decreased(P＜0.05),and the expression level of BCL-2 protein was significantly increased(P＜0.05).The proportion of microglia(IBA1 positive)and astrocytes(GFAP posi-tive)decreased(P＜0.05).Conclusions BMSCs potentially relieve the detrimental effects of simulated micro-gravity on mouse brain by inhibiting inflammation,apoptosis and cellular senescence.关键词
间充质干细胞(MSCs)/微重力/后肢去负荷/凋亡/细胞衰老Key words
mesenchymal stem cells/microgravity/hindlimb unloading/apoptosis/cellular senescence分类
医药卫生引用本文复制引用
龚金涛,厉建伟,李玉恒,李堑,赵春华..骨髓间充质干细胞缓解模拟微重力对小鼠大脑皮质的不利影响[J].基础医学与临床,2024,44(6):772-778,7.基金项目
中国医学科学院医学与健康科技创新工程(2022-I2M-1-012) （2022-I2M-1-012）
全国重点实验室专项经费(2060204) （2060204）
国家重点研发计划(2020YFA0113000) （2020YFA0113000）
高等学校学科创新引智计划(B18007) （B18007）
