基础医学与临床2024，Vol.44Issue(6)：779-785,7.DOI:10.16352/j.issn.1001-6325.2024.06.0779
沙门氏菌感染巨噬细胞的动态转录物组分析
Dynamic transcriptomic analysis of macrophages infected with Salmonella typhimurium
摘要
Abstract
Objective To comprehensively understand the dynamic transcriptional landscape during infection through investigating the temporal molecular changes in macrophages RAW 264.7 upon infection with Salmonella typhimurium SL1344.Methods Macrophages RAW 264.7 were infected with Salmonella typhimurium SL1344,and cell samples were collected at 0 h,8 h,and 16 h for RNA-sequencing(RNA-seq).Upstream and downstream analyses of the transcriptome data including differential gene expression,clustering,functional annotation,and mo-lecular network studies were conducted to elucidate the signaling pathways changes in macrophages.Results Infec-ted macrophages exhibited significant morphological and transcriptional changes.Differential gene analysis identified significant upregulation and downregulation patterns.Clustering revealed six gene clusters involving various signaling pathways,such as immune response,membrane transport,and lipid catabolic process.Conclusions Macrophages dynamically respond to Salmonella typhimurium infection,displaying distinct temporal gene expression patterns.The coordinated activation of immune response,membrane transport,and lipid catabolic process pathways implies a multifaceted cellular adaptation to external infections,providing essential insights into the molecular mechanisms of macrophage response to Salmonella typhimurium infection.关键词
巨噬细胞/感染模型/免疫反应/转录物组/时序分析Key words
macrophages/infection model/immune response/transcriptome/temporal analysis分类
生物科学引用本文复制引用
宋博渊,吴雪丽,李雪媛,王莉莎,陈阳..沙门氏菌感染巨噬细胞的动态转录物组分析[J].基础医学与临床,2024,44(6):779-785,7.基金项目
中国医学科学院中央级公益性科研院所基本科研业务费(2021-RC310-007) （2021-RC310-007）
