鲍曼不动杆菌基因突变文库构建及耐药抑制基因筛查与鉴定OA北大核心CSTPCD
Construction of gene mutation library of Acinetobacter baumannii and screening and identification of drug resistance suppressor genes
为挖掘鲍曼不动杆菌(Acinetobacter baumannii)耐药性产生的调控基因,以敏感株鲍曼不动杆菌AB43为研究对象,采用Tn5转座突变技术建立突变库并构建亚胺培南(IPM)和四环素(Tet)抗生素筛选模型,通过全基因组测序确认耐药突变株的基因突变位点.结果表明:成功地从突变库的4 000余株突变株中筛选出6株对亚胺培南耐药性升高的突变株,1株对四环素耐药性升高的突变株.通过全基因组测序确认,UvrD、LipA、CzcD、OprD等基因突变可导致鲍曼不动杆菌AB43耐药性、生物膜形成能力和生长速率发生改变.这一研究为进一步探索鲍曼不动杆菌耐药调控机制奠定了基础.
In order to explore the regulatory genes regulating drug resistance of Acinetobacter baumannii,the mutant library of sensitive A.baumannii strain AB43 was established by Tn5 transposable mutation technique,and the antibiotic screening models of imipenem(IPM)and tetracycline(Tet)were constructed.Finally,the gene mutation sites of drug resistant mutants were identified by whole genome sequencing.The results showed that six mutants with increased resist-ance to imipenem and one mutant with increased resistance to tetracycline were successfully screened from more than 4 000 mutants in the mutation bank.Genome-wide sequencing confirmed that mutations in UvrD,LipA,CzcD,OprD and other genes led to changes in AB43 resistance,biofilm formation ability and growth rate of A.baumannii.The results of this study lay a foundation for further exploring the regulation mechanism of drug resistance of A.baumannii.
苏峻锋;张鹏宇;於亭;徐悦;秦骜;刘翠翠;李国才
扬州大学医学院/江苏省非编码RNA重点实验室/江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州 225009
畜牧业
鲍曼不动杆菌耐药性转座子调控基因
Acinetobacter baumanniidrug resistancetransposonregulatory gene
《扬州大学学报（农业与生命科学版）》 2024 (002)
基于辣椒素与粘菌素联用探讨其对粘菌素耐药鲍曼不动杆菌的作用及其机制
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国家自然科学基金资助项目(82373637、82073611、82002186);江苏省自然科学基金资助项目(BK20231241)
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