扬州大学学报（农业与生命科学版）2024，Vol.45Issue(2)：61-68,8.DOI:10.16872/j.cnki.1671-4652.2024.02.007
鲍曼不动杆菌基因突变文库构建及耐药抑制基因筛查与鉴定
Construction of gene mutation library of Acinetobacter baumannii and screening and identification of drug resistance suppressor genes
摘要
Abstract
In order to explore the regulatory genes regulating drug resistance of Acinetobacter baumannii,the mutant library of sensitive A.baumannii strain AB43 was established by Tn5 transposable mutation technique,and the antibiotic screening models of imipenem(IPM)and tetracycline(Tet)were constructed.Finally,the gene mutation sites of drug resistant mutants were identified by whole genome sequencing.The results showed that six mutants with increased resist-ance to imipenem and one mutant with increased resistance to tetracycline were successfully screened from more than 4 000 mutants in the mutation bank.Genome-wide sequencing confirmed that mutations in UvrD,LipA,CzcD,OprD and other genes led to changes in AB43 resistance,biofilm formation ability and growth rate of A.baumannii.The results of this study lay a foundation for further exploring the regulation mechanism of drug resistance of A.baumannii.关键词
鲍曼不动杆菌/耐药性/转座子/调控基因Key words
Acinetobacter baumannii/drug resistance/transposon/regulatory gene分类
农业科技引用本文复制引用
苏峻锋,张鹏宇,於亭,徐悦,秦骜,刘翠翠,李国才..鲍曼不动杆菌基因突变文库构建及耐药抑制基因筛查与鉴定[J].扬州大学学报（农业与生命科学版）,2024,45(2):61-68,8.基金项目
国家自然科学基金资助项目(82373637、82073611、82002186) （82373637、82073611、82002186）
江苏省自然科学基金资助项目(BK20231241) （BK20231241）
