扬州大学学报（农业与生命科学版）2024，Vol.45Issue(2)：69-75,7.DOI:10.16872/j.cnki.1671-4652.2024.02.008
牡蛎共生菌Myceliophthora lutea ML-1发酵液的化学成分研究
Chemical constituents from the fermentation broth of Myceliophthora lutea ML-1 associated with Concha ostreae
摘要
Abstract
In order to study the chemical constituents from the liquid culture of Myceliophthora lutea ML-1 associated with Concha ostreae,isolation and purification of the crude extract of the broth of M.lutea ML-1 were performed firstly through silica gel column chromatography,Sephadex LH-20 gel column chromatography,C18 reversed-phase column chromatography and HPLC,etc.Then the structures of obtained compounds were elucidated by modern spectroscopic techniques such as HR-MS and NMR spectroscopy and comparison with literatures.The chemical substances in the crude extract of the liquid culture of M.lutea ML-1 were also analyzed by LC-MS/MS molecular networking.Five compounds were isolated from the fermentation broth of M.lutea ML-1,which were identified as 3-methoxy-5-pentadecylphenol(1),3-methoxy-5-heptadecylphenol(2),3-methoxy-5-nonadecylphenol(3),3-methoxy-5-heneicosylphenol(4)and uracil(5),respectively.Molecular networking(MN)analysis showed that the fermentation broth of M.lutea ML-1 was rich in vari-ous chemical constituents,in addition to resorcinol compounds,it also contained alkaloids such as cyclic dipeptides,am-ides and indoles.Compounds 1-4 isolated from M.lutea ML-1 associated with Concha ostreae were 5-alkyl-3-methoxy-phenol compounds,and MN analysis result provides an idea for the subsequent directed separation of compounds.关键词
牡蛎/共生菌/Myceliophthora lutea/次生代谢产物/分子网络Key words
Concha ostreae/symbiotic microorganism/Myceliophthora lutea/secondary metabolite/molecular networking分类
医药卫生引用本文复制引用
陶超,袁梦,朱小丽,郝增君,王凤舞,申丽..牡蛎共生菌Myceliophthora lutea ML-1发酵液的化学成分研究[J].扬州大学学报（农业与生命科学版）,2024,45(2):69-75,7.基金项目
山东省自然科学基金资助项目(ZR2020MB101) （ZR2020MB101）
扬州大学大学生科创基金项目(X20220741) （X20220741）
