扬州大学学报（农业与生命科学版）2024，Vol.45Issue(2)：93-101,9.DOI:10.16872/j.cnki.1671-4652.2024.02.011
番茄灰霉病生防细菌QZ-1生防潜力研究
Study on biocontrol potential of antagonistic bacterium QZ-1 against tomato Botrytis cinerea
摘要
Abstract
In order to clarify the biocontrol potential of strain QZ-1 against tomato gray mold,the antagonistic bacteri-um QZ-1,isolated from the rhizosphere soil of peach trees in Qingzhou,Shandong Province,the antifungal activity was determined.The antagonistic effects of strain QZ-1 on various phytopathogenic fungi and the control effect against tomato gray mold were assayed using plate confrontation assays and fruit inoculation tests.The results showed that strain QZ-1 dis-played different degrees of inhibitary ability against tested plant pathogenic fungi.Moreover,the sterile filtrate of QZ-1 could sig-nificantly inhibit mycelial growth of B.cinerea,Phomopsis amygdali and Sclerotinia sclerotiorum,and the inhibition rates were 80.00％,77.33％and 76.67％respectively.QZ-1 also exhibited a good biological control effect to B.cinerea in tomato fruit.Strain QZ-1 displayed 99％similarities with that of Bacillus velezensis and B.amylolique faciens based on the 16S rDNA and rpoB sequence analysis.Combined with the physiological and biochemical traits,the strain QZ-1 was identified as B.velezensis.PCR amplification results showed that the presence of 1 203 bp ituD and 1 400 bp fenB genes,coding for iturin A and fengycin,in the genomic DNA of QZ-1.Mass spectrometry analysis suggested that the iturin A and its homologues were the main antimicrobial compounds produced by QZ-1.In summary,B.velezensis QZ-1 effectively inhibits the mycelia development of B.cinerea through the iturin A production,and provides an excellent new biocontrol resource for controlling of tomato gray mold.关键词
番茄灰霉病/贝莱斯芽胞杆菌/伊枯草菌素/生物防治Key words
Botrytis cinerea/Bacillus velezensis/iturin/biological control分类
农业科技引用本文复制引用
宋晓雅,毛子玥,温德宇,朱宇昕,张清霞..番茄灰霉病生防细菌QZ-1生防潜力研究[J].扬州大学学报（农业与生命科学版）,2024,45(2):93-101,9.基金项目
国家自然科学基金资助项目(32072471) （32072471）
扬州市科技计划(现代农业)项目(YZ2023056) （现代农业）
