解剖学杂志2024，Vol.47Issue(1)：24-28,5.DOI:10.3969/j.issn.1001-1633.2024.01.005
核纤层蛋白基因在胶质母细胞瘤恶性生物学行为中的作用
Role of lamin A/C gene in the malignant biological behavior of glioblastoma
摘要
Abstract
Objective:To study the expression characteristics of lamin A/C(LMNA)gene in human glioblastoma and the mechanisms of its roles in the pathogenesis of glioblastoma.Methods:The study analyzed the expression of LMNA gene in glioblastoma and its correlation with patient prognosis in the public databases of TCGA.The LMNA gene sh-RNA virus was constructed to generate stable LMNA knockdown strains of glioma cell line U87.Then the clone formation and 3D spheroidization experiments were conducted on the LMNA knockdown strains of U87 and its control group to detect the impact of LMNA gene on cell proliferation and spheroidization ability.The resistance of LMNA knockdown U87 to temozolomide was detected through temozolomide treatment assay.The inhibitory effect of LMNA knockdown on glioblastoma growth and proliferation in vivo was detected through intracranial tumor implantation experiments in nude mice.Results:TCGA data showed that the expression level of LMNA in glioblastoma tissue was significantly higher than that in normal tissue,and it was negatively correlated with patient survival prognosis.The expression level of LMNA was positively correlated with the malignancy of tumor cells.LMNA knockdown significantly reduced the clone generation and spheroidization ability of U87 cell line,while it significantly increased the sensitivity of U87 cell line to temozolomide.The intracranial tumor implantation experiments in nude mice showed that the U87 cell line with LMNA knockdown significantly reduced the tumor volume in the mouse brain and improved their survival time.Conclusions:LMNA gene expression is increased in glioblastoma and negatively correlated with patient survival.LMNA gene knockdown can inhibit the proliferation of U87 glioma cell line and increase its sensitivity to temozolomide,making it as a potential target for glioblastoma treatment.关键词
核纤层蛋白/核纤层蛋白基因/胶质母细胞瘤/替莫唑胺Key words
lamin A/C/lamin A/C gene/glioblastoma/temozolomide分类
医药卫生引用本文复制引用
王鹏,樊文科,朱克明,卓恺明,咸伟伟..核纤层蛋白基因在胶质母细胞瘤恶性生物学行为中的作用[J].解剖学杂志,2024,47(1):24-28,5.基金项目
上海交通大学医工交叉研究基金(YG2017MS43) （YG2017MS43）
