解剖学杂志2024，Vol.47Issue(1)：40-44,5.DOI:10.3969/j.issn.1001-1633.2024.01.008
过表达同源盒A5对人甲状腺癌细胞增殖的影响及作用机制
Effect of overexpression of HOXA5 on proliferation of human thyroid cancer cells and its mechanism
田荣英 1王缚鲲 1安黎云 1李芳 1张会峰 1郑运周 1冉向阳1
作者信息
- 1. 中国人民解放军联勤保障部队第980医院检验科,石家庄 050081
- 折叠
摘要
Abstract
Objective:To investigate the effect of homeobox A5(HOXA5)on the biological function of human thyroid cancer TPC1 cells and its possible mechanism.Methods:The expression of HOXA5 mRNA and protein in TPC1 cells was analyzed by RT-qPCR and Western blotting.Negative control plasmid(OE-NC)and HOXA5 overexpression plasmid(OE-HOXA5)were transfected into TPC1 cells using lentiviral transduction and the transfection efficiency was verified.Cell proliferation,colony forming ability and cell cycle were detected by CCK-8,colony formation assay and flow cytometry,respectively.The expression of proliferation related proteins and AKT pathway related proteins were detected by Western blotting.Results:The expression levels of HOXA5 mRNA and protein in thyroid cancer cells were significantly lower than that in thyroid follicular epithelial cells.After up-regulation of HOXA5 expression,the proliferation and clonogenesis of TPC1 cells were significantly inhibited,and the percentage of cells in G0/G1 phase was significantly increased,but the percentage of cells in S phase was decreased.The expression levels of proliferation-related proteins Ki67,c-Myc,as well as key proteins in the AKT signaling pathway,p-GSK3β,and p-AKT were significantly down-regulated.Conclusion:HOXA5 is down-regulated in thyroid cancer,and overexpression of HOXA5 gene can inhibit the proliferation and clonogenesis of thyroid cancer cells,and its mechanism may involve the activation of AKT signaling pathway.关键词
同源盒A5/甲状腺癌/细胞增殖/细胞周期/AKT信号通路Key words
HOXA5/thyroid carcinoma/cell proliferation/cell cycle/AKT signaling pathway分类
医药卫生引用本文复制引用
田荣英,王缚鲲,安黎云,李芳,张会峰,郑运周,冉向阳..过表达同源盒A5对人甲状腺癌细胞增殖的影响及作用机制[J].解剖学杂志,2024,47(1):40-44,5.
