西南医科大学学报2024，Vol.47Issue(3)：200-205,6.DOI:10.3969/j.issn.2096-3351.2024.03.004
厌氧菌介导的肿瘤微环境响应型纳米粒子增强肺癌疗效
Anaerobic Bacteria-Mediated Tumor Microenvironment-Responsive Nanopar-ticles Enhance the Therapeutic Efficacy of Lung Cancer
摘要
Abstract
Objective Traditional chemotherapy drugs are unable to maintain high concentration in the tumor,which leads to the reduction of clinical efficacy of lung cancer and serious side effects.The purpose of this study is to develop a novel nanodrug mediated by anaerobic bacteria to increase the drug concentration in tumor tissue and improve the therapeutic effect.Methods A degradable poly-mer was synthesized and doxorubicin nanoparticles(Ab-DOX-s-NPS)modified with Bifidobacterium infantis(Bi)antibody were pre-pared.The morphology,drug release behavior and cell uptake were characterized in vitro.An A549 lung cancer-bearing mouse model was established to evaluate the effect of the nanoparticles on inhibiting tumor growth and to investigate their toxic and side effects.Re-sults The transmission electron microscope(TEM)image and gel electrophoresis protein map confirmed the successful preparation of Ab-DOX-s-s-NPS nanoparticles,which could combine well with bacteria.It could rapidly release drugs and be absorbed by tumor cells in high GSH environment.With the pre-implanted bifidobacterium as the target,the nanoparticles could actively enrich to the an-oxic regions of tumor.It could significantly inhibit the growth of tumor and prolong the survival time of mice.Compared with the myocar-dial fibrosis caused by free adriamycin,the nanodrug did not cause significant cardiac toxicity and liver and kidney function damage.Conclusion The nanoparticles prepared in this study were reduction-responsive and could be actively targeted and enriched into tumor tissue through the recruitment of Bi,which had excellent anti-tumor effect on lung cancer transplanted tumor.The drug system was ex-pected to become a new strategy for the treatment of other malignant solid tumors.关键词
婴儿双歧杆菌/肿瘤乏氧/纳米粒子/多柔比星/肺癌Key words
Bifidobacterium infantis/Tumor hypoxia/Nanoparticles/Doxorubicin/Lung cancer分类
医药卫生引用本文复制引用
李月,熊康,卢韵,傅少志..厌氧菌介导的肿瘤微环境响应型纳米粒子增强肺癌疗效[J].西南医科大学学报,2024,47(3):200-205,6.基金项目
四川省科技厅应用基础研究项目(2020YJ0385) （2020YJ0385）
西南医科大学应用基础重点项目(2021ZKZD014) （2021ZKZD014）
