西南医科大学学报2024，Vol.47Issue(3)：210-214,5.DOI:10.3969/j.issn.2096-3351.2024.03.006
血管生成素-2通过膜突蛋白介导内皮细胞通透性增高
Angiopoietin-2 increases endothelial cell permeability through moesin mediation
摘要
Abstract
Objective To probe into the functions of angiopoietin-2(Ang-2)in endothelial cell permeability and the potential molecular mechanisms involved.Methods The endothelial cells were plated onto transwell,followed by stimulation with Ang-2(200 ng/mL)and then the endothelial cell permeability were detected.The endothelial cells cultured in 6-well plates were stimulated with Ang-2(200 ng/mL)for 0,15,30 and 60 min.Then the moesin phosphorylation was detected by western blotting.Moreover,the moesin siRNA was used to knock down the expression of moesin and the effects of moesin knockdown on Ang-2-regulated endothelial cell per-meability,stress fiber formation and vascular endothelial cadherin(VE-cadherin)were detected.Results Ang-2 significantly in-creased endothelial cell permeability in a time-dependent manner.The activation of moesin was remarkably evoked with Ang-2 treat-ment for 15 min and peaked in 30 min.However,the anti-Tie-2 antibody significantly prevented Ang-2-induced moesin phosphoryla-tion.Furthermore,knockdown of moesin with moesin siRNA significantly prevented Ang-2-induced endothelial cell permeability in-crease,stress fiber formation and VE-cadherin disruption.Conclusion Ang-2 increased endothelial cell permeability through moesin mediation.关键词
血管生成素-2/膜突蛋白/内皮细胞通透性Key words
Angiopoietin-2/Moesin/Endothelial cell permeability分类
医药卫生引用本文复制引用
袁双双,余琴,邓鑫,游婧璨,李永杰,陈妮,王立群..血管生成素-2通过膜突蛋白介导内皮细胞通透性增高[J].西南医科大学学报,2024,47(3):210-214,5.基金项目
国家自然科学基金(81970260) （81970260）
四川省自然科学基金项目(2022NSFSC0671) （2022NSFSC0671）
