西南医科大学学报2024，Vol.47Issue(3)：221-225,5.DOI:10.3969/j.issn.2096-3351.2024.03.008
基于液相色谱-串联质谱的激素测定平台助力多囊卵巢综合征的精准诊疗
Liquid chromatography-tandem mass spectrometry-based hormone measure-ment platform facilitates accurate diagnosis and treatment of polycystic ovary syndrome
摘要
Abstract
Objective A hormone determination platform based on liquid chromatography-tandem mass spectrometry(LC-MS/MS)was established to compare the levels of serum testosterone(T),dehydroepiandrosterone sulfate(DHEA-S),progesterone(P4),and estradiol(E2)with those measured by CLIA.This comparison clarified the clinical value of the LC-MS/MS platform for the diagno-sis of hyperandrogenism and polycystic ovary syndrome(PCOS).Methods 35 patients with PCOS and 33 normal controls were in-cluded.Serum levels of testosterone(T),4-androstenedione(A4),dehydroepiandrosterone sulfate(DHEA-S),dihydrotestosterone(DHT),dehydroepiandrosterone(DHEA),progesterone(P4),estradiol(E2),estrone(E1),estriol(E3),17-hydroxyprogesterone(17-OHP),cortisol,and cortisone were measured by LC-MS/MS and compared with CLIA-measured levels of serum T,DHEA-S,P4,and E2.The area under the curve,sensitivity,specificity,and Jordon's index,as well as the positive predictive value and negative pre-dictive value of ROC curves,were analyzed to diagnose PCOS using serum T,A4,and E1 levels measured by LC-MS/MS.Results The levels of serum T,DHEA-S,P4 and E2 measured by LC-MS/MS were lower than those of CLIA,the differences were statistically signifi-cant(P<0.05).The detection rate of hyperandrogenism could be improved by the combined detection of T,A4,DHEA-S,DHT and DHEA by LC-MS/MS(P<0.05).The serum levels of T,A4 and E1 in patients with PCOS were higher than that of the normal controls(P<0.05).The ROC curves also suggested that the determination of serum T,A4 and E1 levels by LC-MS/MS were valuable for the di-agnosis of PCOS(P<0.05).Conclusions The LC-MS/MS hormone assay platform could improve the detection rate of multiple andro-gens,enhancing the diagnosis of hyperandrogenemia and better identifying PCOS patients compared to CLIA.关键词
多囊卵巢综合征/液相色谱-串联质谱法/化学发光免疫分析法/高雄激素血症/睾酮/双氢睾酮Key words
Polycystic Ovary Syndrome/Liquid Chromatography-Tandem Mass Spectrometry/Chemiluminescence Immunoas-say/Hyperandrogenism/Testosterone/Dihydrotestosterone分类
医药卫生引用本文复制引用
李凯俊,张燊,何帆..基于液相色谱-串联质谱的激素测定平台助力多囊卵巢综合征的精准诊疗[J].西南医科大学学报,2024,47(3):221-225,5.基金项目
国家自然科学基金(82101710) （82101710）
