西南医科大学学报2024，Vol.47Issue(3)：231-235,5.DOI:10.3969/j.issn.2096-3351.2024.03.010
二联体亲子鉴定罕见案例分析及应对策略
Analysis and solution of a rare case of parentage testing of duos
摘要
Abstract
Objective This study aimed to explore an efficient method in a rare case of parentage testing of duos.Method DNA extraction was performed on rare case samples,and parentage testing of duos and trios was performed using AGCU EX-22 Kit,and par-entage testing of duos was also performed using Microreader 36A Kit.The electrophoretic analysis was conducted in the genetic ana-lyzer.Then the data were analyzed with GeneMapper ID-X to get STR profiling and corresponding locus data,and the combined parent-age index(CPI)was calculated.Results Father-child parentage testing of duos was conducted using AGCU EX-22 Kit.The CPI result was 7.0516×102,greater than 0.0001 yet less than 10 000.According to the"Specification of Parentage Testing"(GB/T 37223-2018),the CPI result neither supported the conclusion that the tested father was the biological father of the tested child,nor did it eliminate this possibility.Therefore,it was not possible to express a clear opinion on this parentage testing of duos.In this case,the DNA sample of the tested child's mother was added in the parentage testing of duos which was changed into a parentage testing of trios using the same test-ing kits.The CPI of the revised test was 2.6327×108,greater than 10 000.This result was positive that the tested father was the biologi-cal father of the tested child and a definite opinion of parentage testing could be issued.At the same time,the number of STR loci was increased and the father-child parentage testing of duos was conducted using the Microreader 36A Test Kit.The CPI value was 1.0378×109,greater than 10 000.Thus,a definite parentage opinion could be issued.Conclusion In parentage testing of duos,either by add-ing the child's biological mother for aparentage testing of trios or by adding the STR loci for testing,could improve the CPI and help to produce a definitive identification opinion in rare and difficult parentage testing.关键词
短串联重复序列/二联体亲权鉴定/三联体亲权鉴定/累积亲权指数Key words
Short tandem repeats/Parentagetesting of duos/Parentage testing of trios/Combined parentage index分类
社会科学引用本文复制引用
郭侃,付杰文,宋炳辉,钱捷,罗章,成竞梁,何涛,傅俊江..二联体亲子鉴定罕见案例分析及应对策略[J].西南医科大学学报,2024,47(3):231-235,5.基金项目
国家自然科学基金(82073263) （82073263）
泸州市科技计划项目(2021-SYF-37) （2021-SYF-37）
四川省自然科学基金(2022NSFSC0737) （2022NSFSC0737）
