西南医科大学学报2024，Vol.47Issue(3)：251-255,5.DOI:10.3969/j.issn.2096-3351.2024.03.014
柴黄清胰活血颗粒对雨蛙素诱导的大鼠胰腺腺泡细胞损伤的保护作用及机制
Investigate the protective effect and mechanism of Chaihuang Qingyi Huoxue Granule on pancreatic acinous cell injury induced by caerulin
摘要
Abstract
Objective To investigate the protective effect and underlying mechanism of Chaihuang Qingyi Huoxue Granule on the JAK2/STAT3 signal pathway in rat pancreatic acinar cells induced by caerulin.Methods The pancreatic acinar cells AR42J were cul-tured and treated with different concentrations of Chaihuang Qingyi Huoxue granules.The effects of different concentrations of Chai-huang Qingyi Huoxue granules on the viability of AR42J cells were detected by CCK-8 assay to determine the best drug concentration.AR42J cells were stimulated with caerulin for 6 hours to mimic acute pancreatitis and randomly divided into the control group,the model group,the low,middle,and high dose groups of Chaihuang Qingyi Huoxue Granule.The contents of TNF-α,IL-6,and IL-1β in culture supernatants were determined by ELISA;The expression of JAK2 and STAT3 genes were detected by real-time quantitative PCR(q-RTPCR);The protein expression levels of JAK2,STAT3,p-JAK2,p-STAT3 were detected by Western blot.All the above da-tas were analyzed using GraphPad Prism 9.0 software and one-way analysis of variance.Results CCK-8 assay showed that the average cell survival rate was more than 100%when the drug concentration were 0.5,1,and 2 mg/L.ELISA showed that compared with the con-trol group,the inflammatory cytokines IL-6,IL-8,and TNF-α were increased in the model group,suggesting the successful establish-ment of acute pancreatitis.The contents of TNF-α,IL-6,and IL-1β in cell supernatants of AR42J cells treated with Chaihuang Qingyi Huoxue Granule group(0.5,1,2 mg/mL)were significantly lower than those in the model group after treatment(P<0.05).The con-tents of STAT3 mRNA in AR42J cell in the Chaihuang Qingyi Huoxue Granule group(0.5,1,2 mg/mL),JAK2 mRNA in AR42J cell in the Chaihuang Qingyi Huoxue Granule group(2 mg/mL)were significantly lower than those in the model group after treatment(P<0.05).The ratio of p-JAK2/JAK2,p-STAT3/STAT3 in AR42J cell in the Chaihuang Qingyi Huoxue Granule group(0.5,1,2 mg/mL)were significantly lower than those in model group after treatment(P<0.05).Conclusion Chaihuang Qingyi Huoxue Granule might re-duce the inflammatory response of AR42J cells by regulating the expression of key molecules in JAK2/STAT3 signaling pathway.关键词
柴黄清胰活血颗粒/JAK2/STAT3信号通路/AR42J细胞/急性胰腺炎Key words
Chaihuang Qingyi Huoxue Granule/JAK2/STAT3 signal path/AR42J cells/Acute pancreatitis分类
中医学引用本文复制引用
付加伟,唐祖鑫,陆俊,邹鑫,李丽,赵龙,李志..柴黄清胰活血颗粒对雨蛙素诱导的大鼠胰腺腺泡细胞损伤的保护作用及机制[J].西南医科大学学报,2024,47(3):251-255,5.基金项目
四川省科技厅项目(2019YFS0163) （2019YFS0163）
四川省中管局项目(2023MS416) （2023MS416）
川大-泸州市政府战略合作项目(2020CDLZ-18) （2020CDLZ-18）
西南医科大学校级课题(2022QN095) （2022QN095）
西南医科大学附属中医医院科研团队创新培育项目(2022-CXTD-01) （2022-CXTD-01）
