负载虾青素对富含DHA的磷脂酰胆碱脂质体膜结构稳定性的影响OA北大核心CSTPCD

In order to investigate the effect of embedded astaxanthin on the stability of phospholipid membranes structure of liposomes,docosahexaenoic acid-enriched phosphatidylcholine(DHA-PC)extracted and purified from squid roe was selected as the liposome membrane material to prepare astaxanthin-embedded DHA-PC liposome(DPC-AST)and blank DHA-PC liposome(DPC)with ethanol injection method.It was found that the mass ratio of DHA-PC to astaxanthin,as well as solvent volume ratio and incubation time were key factors to control the form of embedded astaxanthin.Astaxanthin J-aggregates can be successfully embedded in lipid bilayers when the mass ratio of DHA-PC to astaxanthin was 1∶0.05 or 1∶0.10,the volume ratio of water to ethanol was 2∶1,and incubation time was 40 min.The particle size(362～386 nm)of DPC-AST embedded with J-aggregates astaxanthin was significantly larger than that of DPC(57 nm).The instability index(0.5)of DPC-AST embedded with J-aggregates astaxanthin was significantly lower than that of DPC(1.2)as determined by multiple light scattering technologies.DPC-AST embedded with J-aggregates astaxanthin had better microstructure stability.The fluorescence polarization analysis showed that the membrane fluidity of DPC-AST is decreased in comparison with that of DPC.Meanwhile,the fine regulation of liposome membrane fluidity could be achieved by changing the embedding form of astaxanthin.Moreover,the improved anti-lipid peroxidation capacity of astaxanthin further proved that astaxanthin can significantly improve the stability of the membrane structure of DHA-PC liposomes.