超高效液相色谱-串联质谱法同时测定地锦草中8种成分的含量OA
Simultaneous Determination of Eight Components in Euphorbiae Humifusae Herba by UPLC-MS/MS
目的:建立超高效液相色谱-串联质谱法(UPLC-MS/MS)用于同时测定地锦草中2种有机酸类和6种黄酮类成分的含量.方法:采用Hypersil GOLD C18(150 mm×3.0 mm,3 μm),流动相为0.3％甲酸溶液(A)-乙腈(B),梯度洗脱,流速为0.3 mL/min,柱温为40 ℃,进样量为2 μL;质谱采用ESI源,负离子模式监测,喷雾电压为-3000 V,离子化温度为500 ℃,多反应监测模式(MRM)用于定量分析.结果:所测…查看全部>>
To develop a UPLC-MS/MSmethod for simultaneous determination of six flavonoids and two organic acids in Euphorbiae Humifusae Herba.Methods:The analysis was performed on a Hypersil GOLD C18 column(150 mm×3.0 mm,3 μm)at 40 ℃.The mobile phase was 0.3％formic acid solution(A)-hexanenitrile(B)in a gradient elution,which was delivered at a flow rate of 0.3 mL/min.The injection volume was 2 μL.For the MS/MS analysis,the ESI source was operated in the negative-ion mo…查看全部>>
郑璇杏;张煜帆;佘溢彬;郑婧婧;郭漫炫
潮州市药品检验所,广东潮州 521000潮州市药品检验所,广东潮州 521000潮州市药品检验所,广东潮州 521000潮州市药品检验所,广东潮州 521000潮州市药品检验所,广东潮州 521000
地锦草UPLC-MS/MS有机酸黄酮含量
Euphorbiae Humifusae HerbaUPLC-MS/MSorganic acidflavonoidcontent
《品牌与标准化》 2024 (3)
37-41,5
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