现代检验医学杂志2024，Vol.39Issue(3)：48-52,108,6.DOI:10.3969/j.issn.1671-7414.2024.03.008
子宫内膜癌组织中dMMR蛋白和miRNA Let-7表达水平及临床价值的研究
Expression Level and Clinical Value of dMMR Proteins and miRNA Let-7 in Endometrial Carcinoma Tissue
摘要
Abstract
Objective To investigate the relationship between the expression level of miRNA Let-7 and the deletion of DNA mismatch repair(dMMR)proteins in endometrial carcinoma(EC).Methods A total of 74 patients with EC who underwent radical surgery at Lianyungang Maternal and Child Health Hospital in Jiangsu Province from May 2016 to December 2022 were selected.Immunohistochemistry was used to detect the expression of dMMR proteins(including MLH1,PMS2,MSH2,and MSH6),while real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was performed to measure the relative expression levelof miRNA Let-7.According to the expression of dMMR proteins,EC patients were divided into the complete expression group(n=43)and the absent expression group(n=31).Logistic multivariate regression analysis was conducted to identify risk factors associated with dMMR protein deficiency,and receiver operating characteristic(ROC)curve was plotted to evaluate the predictive value of relevant factors.Results In 74 cases of EC,the expression level of miRNA Let-7 was higher in the myographic infiltration＜1/2 group than in the myographic infiltration ≥ 1/2 group,and the difference was significant(t=1.79,P=0.04).The deletion rate of dMMR protein expression was 41.89％,the deletion rate in patients aged＜55 years and miRNA Let-7 low expression group(＜0.715)were higher than those in ≥ 55 years and miRNA Let-7 high expression group(≥ 0.715),with significant differences(x2=3.92,4.50,all P＜0.05).Logistic regression analysis revealed that miRNA Let-7 expression level was an independent risk factor for dMMR expression deletion(P=0.012).Spearman correlation analysis demonstrated a negative correlation between miRNA Let-7 expression level and dMMR protein deletion(r=-0.247,P=0.034).ROC curve analysis indicated that the expression level of miRNA Let-7 had a certain predictive value for dMMR protein deletion in EC patients,with an AUC of 0.737,an optimal critical value of 0.77,a sensitivity and a specificity of 0.651 and 0.806,respectively.Conclusion The expression level of miRNA Let-7 was correlated with the deletion of dMMR proteins in patients diagnosed with EC.Moreover,it served as a potential risk factor for dMMR protein deficiency.It may offer valuable insights into predicting dMMR expression deletion.关键词
子宫内膜癌/林奇综合征/DNA错配修复蛋白/微小RNA Let-7Key words
endometrial carcinoma/Lynch syndrome/DNAmismatch repair proteins/miRNA Let-7分类
医药卫生引用本文复制引用
张亚军,刘裴丽,江月,闫莉,陈云云,蒋欣,孟凡菲..子宫内膜癌组织中dMMR蛋白和miRNA Let-7表达水平及临床价值的研究[J].现代检验医学杂志,2024,39(3):48-52,108,6.基金项目
江苏省连云港市卫生计生科技项目(编号:ZD202005). （编号:ZD202005）
