摘要
Abstract
Objective To investigate the correlation between serum microRNA(miR)-27a-3p and homeobox C6(HOXC6)levels expression and coronary artery disease in patients with hypertension complicated by coronary atherosclerotic heart disease(CHD),and provide some references for the prevention and treatment of hypertension complicated with coronary heart disease.Methods A total of 246 hypertensive patients accepted by Beidahuang Group General Hospital from June 2021 to June 2023 were collected as the study subjects,with 129 hypertensive patients complicated with coronary heart disease as the concurrent group and 117 simple hypertensive patients as the non concurrent group.Quantitative real time fluorescent PCR(qRT-PCR)was applied to detect the expression levels of miR-27a-3p and HOXC6 in serum.Spearman method was applied to analyze the correlation between serum miR-27a-3p and HOXC6 levels and coronary artery disease in hypertensive patients with coronary heart disease.Multivariate logistic regression was applied to analyze the influencing factors of hypertension complicated with coronary heart disease.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum miR-27a-3p and HOXC6 levels for hypertension complicated with coronary heart disease.Results The course of hypertension(6.69±1.35年),total cholesterol(TC)(5.96±0.86mmol/L)and low-density lipoprotein cholesterol(LDL-C)(3.36±0.51mmol/L)in the concurrent group were higher than those in the non concurrent group(5.87±1.32 年,5.42±0.67mmol/L,2.98±0.48mmol/L),the serum levels ofmiR-27a-3p(1.35±0.34)andHOXC6(1.29±0.33)in the concurrent group were higher than those in the non concurrent group(1.06±0.23,1.03±0.25),and the differences were statistically significant(t=4.808,5.454,6.001,7.755,6.911,all P＜0.05).The expression levels of miR-27a-3p(0.54±0.13,1.19±0.31,2.18±0.55)and HOXC6(0.47±0.12,1.12±0.31,2.13±0.52)in mild,moderate and severe groups were gradually increased,and the differences were significant(F=191.026,191.026,all P＜0.05).There were positive correlations between serum miR-27a-3p,HOXC6,and SYNTAX scores in patients with hypertension complicated by coronary heart disease(r=0.563,0.517,all P＜0.05).Serum miR-27a-3p[OR(95％CI):1.627(1.177～2.248)]and HOXC6[OR(95％CI):1.715(1.069～2.750)]were independent risk factors for hypertension complicated with coronary heart disease(all P＜0.05).The combination of miR-27a-3p and HOXC6 levels had an area under the curve(AUC)of 0.896(95％CI:0.851～0.931)for predicting hypertension complicated with coronary heart disease,which was superior to their respective individual diagnoses(Z=2.133,2.815,all P＜0.05).Conclusion The serum levels of miR-27a-3p and HOXC6 in patients with hypertension complicated by coronary heart disease were increased,which was closely related to coronary artery disease.The combination of the two has a high predictive value for hypertension complicated with coronary heart disease.关键词
微小核糖核酸-27a-3p/同源异型盒C6/高血压/冠状动脉粥样硬化性心脏病/冠状动脉病变Key words
micro RNA-27a-3p/homeobox C6/hypertension/coronary atherosclerotic heart disease/coronary artery disease分类
医药卫生
