老年性白内障患者泪液中miR-223和miR-143-3p水平表达与术后干眼症的相关性研究OACSTPCD

Objective To investigate the correlation between postoperative dry eye syndrome and micro RNA(miR)-223 and miR-143-3p factors in tears of elderly cataract patients.Methods From May 2022 to February 2023,92 elderly cataract patients who underwent cataract surgery in Department of Ophthalmology,Guigang Donghui Hospital were regarded as the study subjects.These subjects were separated into a dry eye group(n=42)and a non dry eye group(n=50)based on the occurrence of dry eyes within 7 days after surgery.Meanwhile,92 healthy individuals who underwent physical examination were collected as the healthy control group.Pearson method was applied to analyze the correlation between the expression levels of miR-223 and miR-143-3p in tears and dry eye diagnostic indicators such as tear film break-up time(BUT),comeal fluorescein staining(FL)score,and tear schirmer's test(SIt).Multivariate logistic regression was applied to analyze the relevant factors affecting the occurrence of dry eye after cataract surgery.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of tear miR-223 and miR-143-3p for the occurrence of dry eye after cataract surgery.Results The BUT(12.27±1.69s)and SIt(9.17±1.66mm)in the non dry eye group were higher than those in the dry eye group(8.95±1.02s,4.36±0.97mm),while FL(0.74±0.11 score)was lower than that in the dry eye group(2.52±0.37score).and the differences were statistically significant(t=1.136,16.546,32.386,all P＜0.05).The expression level of miR-223(0.87±0.08)in tears of patients in the dry eye group was lower than that of the healthy control group(1.02±0.03)and the non dry eye group(0.92±0.13),while the expression level of miR-143-3p(1.37±0.32)in tears was opposite(1.01±0.02,1.15±0.26),and the differences were statistically significant(t=15.772,2.170;10.793,3.638,all P＜0.05).The expression level of miR-223 in tears was positively correlated with BUT and SIt(r=0.587,0.503,all P＜0.001),and was negatively correlated with FL(r=-0.442,P＜0.001).The expression level of miR-143-3p in tears negatively correlated with BUT and SIt(r=-0.714,-0.549,all P＜0.001),and was positively correlated with FL(r=0.667,P＜0.001).The proportions of patients with a history of angular conjunctival disease and a surgical incision length of ≥ 3mm in the dry eye group were higher than that in the non dry eye group(x2=12.583,6.505,all P＜0.05).The expression level of miR-223 in tears of patients in the dry eye group was lower than that of the non dry eye group,while the expression level of miR-143-3p was higher than that of the non dry eye group(t=2.170,3.638,all P＜0.05).A history of conjunctival disease(OR=1.982),surgical incision length(OR=2.036),and miR-143-3p(OR=1.653)were risk factors for postoperative dry eye in cataract patients,while miR-223(OR=0.574)was a protective factor(P＜0.05).The areas under the curve(AUC)of tear miR-223 and miR-143-3p detection alone predicted the occurrence of dry eye after cataract surgery were 0.692 and 0.719,respectively,while the AUC of their combined detection was 0.880.The combined detection of the two was superior to miR-223 and miR-143-3p detection alone(Z=3.869,3.810,all P＜0.001).Conclusion The occurrence of dry eye in postoperative elderly cataract patients was related to the expression levels of miR-223 and miR-143-3p in tears.