现代检验医学杂志2024，Vol.39Issue(3)：84-89,6.DOI:10.3969/j.issn.1671-7414.2024.03.014
妊娠期糖尿病患者血清LncRNA DANCR和miR-33a-5p水平表达对妊娠结局预测价值研究
Study on the Predictive Value of Serum LncRNA DANCR and miR-33a-5p Levels in Patients with Gestational Diabetes Mellitus on Pregnancy Outcome
摘要
Abstract
Objective To investigate predictive value of serum levels of long noncoding RNA differentiation antagonizes non-protein coding RNA(LncRNA DANCR)and microRNA(miR)-33a-5p in patients with gestational diabetes mellitus(GDM)on pregnancy outcome.Methods A total of 154 GDM patients who underwent prenatal examination and delivery in the Fourth People's Hospital of Hengshui from August 2021 to February 2023 were collected as the GDM group,and these patients were grouped into a good outcome group of 115 cases and an adverse outcome group of 39 cases based on pregnancy outcomes.Meantime,149 healthy pregnant women with normal glucose tolerance test for 24 weeks were collected as the control group.Realtime fluorescence quantitative PCR(qRT-PCR)method was applied to detect serum LncRNA DANCR and miR-33a-5p levels,and receiver operating characteristic(ROC)curve was used to analyze the value of serum LncRNA DANCR and miR-33a-5p in predicting adverse pregnancy outcomes in GDM patients.Pearson correlation was applied to analyze the correlation among serum LncRNA DANCR,miR-33a-5p,fasting bloodglucos(FBG),fasting insulin(FINS),and homeostasis model assessment of insulin resistance(HOMA-IR)in patients with GDM adverse pregnancy outcomes.Logistic regression was used to analyze the influencing factors of adverse pregnancy outcomes in GDM patients.Results The serum LncRNA DANCR level(0.69±0.15)in the GDM group was lower than that in the control group(1.01±0.22),while the level of miR-33a-5p(1.59±0.40)and the total incidence of adverse pregnancy outcomes(25.34％)were higher than those in the control group(1.02±0.23,5.36％),with significant differences(t/x2=14.835,15.140,23.011,all P＜0.05).The serum LncRNADANCR level(0.50±0.14)in the adverse outcome group was lower than that in the good outcome group(0.75±0.18),while miR-33a-5p(2.00±0.58),FBG(8.97±0.66mmol/L),FINS(18.63±1.31pmol/ml)and HOMA-IR(7.42±0.98)were higher than those in the good outcome group(1.45±0.26,8.01±0.59mmol/L,14.32±1.29pmol/ml,5.10±0.86),the differences were statistically significant(t=7.895～17.961,all P＜0.05).The areas under curve(AUC)of predicting adverse pregnancy outcomes in GDM patients with LncRNA DANCR and miR-33a-5p alone and in combination were 0.820,0.819 and 0.897,respectively.For patients with GDM adverse pregnancy outcomes,serum LncRNA DANCR was negatively correlated with FBG,FINS and HOMAIR(r=-0.498,-0.513,-0.509,allP＜0.05),while miR-33a-5p was positively correlated with FBG,FINS and HOMA-IR(r=0.517,0.494,0.507,all P＜0.05).LncRNA DANCR was a protective factor that affected adverse pregnancy outcomes in GDM patients(OR=0.804,95％CI:0.693～0.933,P=0.004);while miR-33a-5p was a risk factor for adverse pregnancy outcomes in GDM patients(OR=2.747,95％CI:1.444～5.225,P=0.002).Conclusion LncRNA DANCR was decreased and miR-33a-5p was increased in GDM patients with adverse pregnancy outcomes,indicating both may be influencing factors for adverse pregnancy outcomes.关键词
妊娠期糖尿病/妊娠结局/长链非编码核糖核酸分化拮抗非蛋白编码RNA/微小RNA-33a-5pKey words
gestational diabetes mellitus/pregnancy outcome/long noncoding RNA differentiation antagonizes nonprotein coding RNA/miR-33a-5p分类
医药卫生引用本文复制引用
王春燕,刘慧赏,马少未..妊娠期糖尿病患者血清LncRNA DANCR和miR-33a-5p水平表达对妊娠结局预测价值研究[J].现代检验医学杂志,2024,39(3):84-89,6.基金项目
衡水市科技计划项目(2022014021Z):妊娠期糖尿病危险因素及其预防价值的分析. （2022014021Z）
