现代检验医学杂志2024，Vol.39Issue(3)：199-204,6.DOI:10.3969/j.issn.1671-7414.2024.03.034
CRISPR-Cas13a系统在病原体检测应用中的最新研究进展
Recent Advances in the Application of CRISPR-Cas13a Systems in Pathogen Detection
摘要
Abstract
As a new generation of molecular diagnostic tools emerged in recent years,the clustered regularly interspaced short palindromic repeats/CRISPR-associated(CRISPR-Cas)systems have been widely used in gene editing,nucleic acid detection,and other fields.Due to its high specificity,high sensitivity,without expensive equipment,simple operation,and inexpensive characteristics in nucleic acid detection,the Cas13a subtype has shown great potential in the field of pathogen detection.This article reviews the mechanism of action of CRISPR-Cas13a,its application in pathogen detection,and related detection methods,and looks forward to the application prospects of Cas13a.To facilitate future investigations on the CRISPR-Cas13a systems and their potential in pathogen detection,this study aims to offer inspiration and serve as a valuable reference.关键词
规律成簇的间隔短回文重复序列及相关蛋白13a系统/病原体/核酸检测Key words
clustered regularly interspaced short palindromic repeats/CRISPR-associated 13a systems/pathogen/nucleic acid detection分类
医药卫生引用本文复制引用
李青松,赵聪平,刘静,杨均,燕思羽,张银河..CRISPR-Cas13a系统在病原体检测应用中的最新研究进展[J].现代检验医学杂志,2024,39(3):199-204,6.基金项目
西南医科大学应用基础项目(NO.2021ZKQN002). （NO.2021ZKQN002）
