山东医药2024，Vol.64Issue(14)：6-9,4.DOI:10.3969/j.issn.1002-266X.2024.14.002
黄芪皂苷Ⅱ对肾透明细胞癌细胞迁移和侵袭能力的抑制作用及其机制
Inhibitory effects of astragaloside Ⅱ on invasion and migration of clear cell renal cell carcinoma
摘要
Abstract
Objective To explore the inhibitory effects of astragaloside Ⅱ(ASⅡ)on invasion and migration of clear cell renal cell carcinoma cells,and to analyze the relationships between the effects and Wnt/β-catenin signaling pathway.Methods Human clear cell renal cell carcinoma cells(786-O)were taken and were randomly divided into three groups:the control group,the ASⅡ group,and the LiCl intervention group.In the control group,only fresh serum was used to re-place the culture medium throughout the experiment without any other treatments;in the ASⅡ group,100 μmol/L ASⅡsolution was added to the cells;in the LiCl intervention group,cells were treated with 10 mmol/L LiCl solution,and after 24 h,they were added with 100 μmol/L ASⅡ solution.The migration ability of the cells in each group was measured by Scratch assay,the invasive ability of the cells was detected by Transwell assay,and epithelial-mesenchymal transition(EMT)-related proteins E-cadherin,N-cadherin,and N-cadherin were detected by Western blotting.Results Com-pared with the control group,the cell migration rate decreased in the ASⅡ group;compared with the ASⅡ group,the cell migration rate increased in the LiCl intervention group(both P<0.01).Compared with the control group,the number of transmembrane cells decreased in the ASⅡ group;compared with the ASⅡ group,the number of transmembrane cells in-creased in the LiCl intervention group(both P<0.01).Compared with the control group,the expression level of E-cad-herin protein increased in the ASⅡ group,while the protein expression levels of N-cadherin,Vimentin and β-catenin de-creased.Compared with the ASⅡ group,the expression level of E-cadherin protein in the LiCl intervention group de-creased,while the protein expression levels of N-cadherin,Vimentin and β-catenin increased(all P<0.01).ConclusionsⅡ can inhibit EMT via regulating Wnt/β-catenin signaling pathway.关键词
黄芪皂苷Ⅱ/肾肿瘤/Wnt/β-catenin信号通路/细胞迁移/细胞侵袭/上皮间充质转化Key words
astragaloside Ⅱ/kidney neoplasms/Wnt/β-catenin signaling pathway/cell migration/cell inva-sion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
赵凯,李勋华,王科..黄芪皂苷Ⅱ对肾透明细胞癌细胞迁移和侵袭能力的抑制作用及其机制[J].山东医药,2024,64(14):6-9,4.基金项目
国家自然科学基金资助项目(31971191). （31971191）
