山东医药2024，Vol.64Issue(14)：14-17,21,5.DOI:10.3969/j.issn.1002-266X.2024.14.004
miR-138对肾癌细胞增殖、迁移和侵袭的影响及其机制
Effects of miR-138 on proliferation,migration,and invasion of renal cancer cells
摘要
Abstract
Objective To observe the effects of miR-138 on proliferation,migration,and invasion of renal cancer cells,and to explore its targeted regulatory effect on cyclin D1(CCND1).Methods Kidney cancer cells 786-O were taken and randomly divided into the control group,miR-138 overexpression group,and miR-138 low expression group,which were transfected with miR-138 null liposome,miR-138 mimic,and miR-138 inhibitor,respectively.CCK-8 assay was used to observe the proliferation of the cells in each group,and the Scratch assay was used to observe the cell migration ability.Transwell assay was used to observe cell invasion ability.The target genes of miR-138 were predicted by StarBase software.The luciferase assay was utilized to determine the targeted regulatory relationship between miR-138 and CCND1.Results Compared with the control group,miR-138 overexpression group showed decreased cell proliferation activity,decreased distance of cell migration and the number of cell invasion,and decreased CCND1 protein expression(all P<0.01).Compared with the control group,the miR-138 low expression group showed elevated cell proliferative activity,increased distance of cell migration and the number of cell invasion,and elevated CCND1 protein expression(all P<0.01).StarBase software predictive result indicated that the 3'non-coding region of CCND1 contained the binding site of miR-138.Luciferase assay showed that miR-138 could regulate CCND1.Conclusion The miR-138 could inhibit the proliferation,migration and invasion of renal cancer cells,and its mechanism might be related to the targeted regulation of CCND1.关键词
微小核糖核酸138/肾癌细胞/细胞增殖/细胞迁移/细胞侵袭/细胞周期蛋白D1Key words
miRNA-138/renal cancer cells/cell proliferation/cell migration/cell invasion/cyclin D1分类
医药卫生引用本文复制引用
张丹,王德昌,李志勇,刘佳,李友宽,关欣..miR-138对肾癌细胞增殖、迁移和侵袭的影响及其机制[J].山东医药,2024,64(14):14-17,21,5.基金项目
云南省教育厅科学研究基金项目(2023J2214). （2023J2214）
