上海海洋大学学报2024，Vol.33Issue(3)：552-561,10.DOI:10.12024/jsou.20230204074
抑制Wnt/β-catenin信号通路对始初小头虫再生的影响
Experiment study on the effect of inhibition of Wnt/β-catenin signaling on the regeneration of Capitella teleta
摘要
Abstract
Wnt/β-catenin signaling plays an important role in regeneration in multiple species such as planaria.The study aimed to investigate the effect of Wnt/β-catenin signaling on the regeneration in the annelid,Capitella teleta.Sustained incubation of samples after amputation in 30 μmol/L XAV-939,an exogenous Wnt/β-catenin signaling small molecular inhibitor,revealed that the regeneration of C.teleta was inhibited,with significantly slower regeneration compared to that in the control group and failure to form a regeneration blastema.Neural and muscle growth during regeneration were also affected by inhibition of Wnt signaling revealed by neural and muscle labeling.Subsequently,EdU labeling indicated that the number of proliferating cells in the XAV 939-treated group was significantly less than that in the control group,suggesting that the suppression of the Wnt/β-catenin signaling may have affected the proliferating cells during the regeneration of C.teleta,which also further demonstrating that the Wnt/β-catenin signaling may be involved in the regeneration of C.teleta,and play an important role in the early stage of regeneration.Comparative studies on the regulation of regeneration by wnt signaling in several typical species have shown that the Wnt signaling is relatively conserved in the whole-body regeneration of Cnidaria and Lophotrochozoa.关键词
Wnt/β-catenin信号通路/环节动物/小头虫/Wnt通路抑制剂/再生/增殖细胞Key words
Wnt/β-catenin signaling pathway/annelid/Capitella teleta/Wnt pathway inhibitor/regeneration/proliferating cells分类
生物科学引用本文复制引用
李倩,张琳琳..抑制Wnt/β-catenin信号通路对始初小头虫再生的影响[J].上海海洋大学学报,2024,33(3):552-561,10.基金项目
国家自然科学基金面上项目(41976088) （41976088）
深圳华大生命科学研究院开放基金(BGIRSZ20210013) （BGIRSZ20210013）
中国科学院B类战略先导科技专项(XDB42000000) （XDB42000000）
青岛海洋科学与技术试点国家实验室山东省专项经费(2022QNLM030004) （2022QNLM030004）
