上海海洋大学学报2024，Vol.33Issue(3)：572-580,9.DOI:10.12024/jsou.20230304121
不同粒径微塑料对中华绒螯蟹幼蟹组织结构及肝胰腺抗氧化能力的影响
Toxic effects of different particle size polystyrene microbeads on juvenile Eriocheir sinensis
摘要
Abstract
Studies on the biological effects of microplastics pollution on aquatic animals mostly focused on the ocean,but the potential effects on freshwater organisms are less known.In this study,the effects of polystyrene(PS)particles with different particle sizes(5 μm,1 μm,100 nm)on juvenile Chinese mitten crab(Eriocheir sinensis)were investigated.Hepatopancreas injury index:alanine aminotransferase(ALT),aspartate aminotransferase(AST);serum immune index:alkaline phosphatase(AKP),acid phosphatase(ACP);Oxidative stress index:malondialdehyde(MDA),catalase(CAT);and neurotoxicity index:acetylcholinesterase(AchE)were analyzed.The pathological changes of gills and hepatopancreas were analyzed by hematoxylin-eosin(H.E)staining.The results showed that exposure to microplastics after 7 d,100 nm group had significantly higher MDA content,CAT activity decreased significantly,the AKP activity of three treatment group were significantly lower,and 1 μm and 100 nm group of ACP activity was significantly reduced.The activities of AST,ALT and AchE gradually decreased with the decrease of particle size and the extension of exposure time.In conclusion,microplastics with different particle sizes caused varying degrees of hepatopancreas injury,oxidative stress and reduced immune capacity in crabs.The toxicity level was ranked as follows:100 nm＞1 μm＞5 μm,indicating that the particle size was one of the most important factors affecting the health of Chinese mitten crabs.This study provides basic data and theoretical basis for further understanding the toxic effects of microplastics on freshwater crustaceans.关键词
聚苯乙烯/中华绒螯蟹/粒径/综合毒性Key words
polystyrene/Eriocheir sinensis/particle size/comprehensive toxicity分类
农业科技引用本文复制引用
杨志刚,张晨晨,江青青,陈阿琴,成永旭,王爱民..不同粒径微塑料对中华绒螯蟹幼蟹组织结构及肝胰腺抗氧化能力的影响[J].上海海洋大学学报,2024,33(3):572-580,9.基金项目
国家自然科学基金(32273154) （32273154）
上海市自然科学基金(22ZR1427300) （22ZR1427300）
财政部和农业农村部国家现代农业产业技术体系(CARS-48) （CARS-48）
上海市研究生教育学会研究课题(ShsgeG202215) （ShsgeG202215）
黄河三角洲人才工程(DYRC20190210) （DYRC20190210）
