新发传染病电子杂志2024，Vol.9Issue(2)：7-10,4.DOI:10.19871/j.cnki.xfcrbzz.2024.02.002
运用基质辅助激光解吸电离飞行时间质谱技术分析分枝杆菌液体培养管污染菌菌群特征
MALDI-TOF MS technique was used to analyze the bacterial community characteristics of mycobacterium contamination in liquid culture tubes
摘要
Abstract
Objective To understand the characteristics of contaminated bacteria in liquid culture of mycobacterium,analyze the causes of contamination,and find ways to reduce pollution.Method Collect sputum and bronchoalveolar lavage fluid samples from the Guangxi Zhuang Autonomous Region Chest Hospital between May 10,2023,and August 25,2023 for liquid culture of Mycobacterium.Using MALDI-TOF MS technology to identify contaminated bacteria produced by liquid culture of Mycobacterium,classify the identified strains statistically,and analyze the microbial community characteristics of contaminated bacteria.Result A total of 864 Mycobacteria were cultured in 6090 respiratory tract samples,the positive rate was 14.19%(864/6090),and the contamination rate was 1.17%(71/6090).The main polluting bacteria were Bacillus and Aspergillus,accounting for 31.08%(23/74)and 24.32%(18/74),respectively.Among the 1429 cases of lavage,279 cases of mycobacterium were cultured,the positive rate was 19.52%(279/1429),and the pollution was 13 cases,contamination rate was 0.91%(13/1429).In 4661 sputum samples,585 strains of mycobacterium were cultured.The culture positive rate was 12.55%(585/4661)and the pollution was 58 cases,contamination rate was 1.24%(58/4661).The positive rate between the two specimens exhibited a statistically significant difference(χ2=43.68,P＜0.05),while no statistically significant difference was observed in the contamination rate(χ2=1.06,P＞0.05).Conclusion The contamination microbial community generated during liquid culture of Mycobacterium is complex,mainly composed of Bacillus and Aspergillus.Therefore,it is necessary to standardize the collection and transportation of specimens,and adopt effective pre-treatment methods to reduce the contamination rate of liquid culture.关键词
污染菌/分枝杆菌/基质辅助激光解吸电离飞行时间质谱Key words
Polluting bacteria/Mycobacterium/Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry分类
医药卫生引用本文复制引用
蓝吉慧,唐柳生,黄葵,刘爱梅,董捷..运用基质辅助激光解吸电离飞行时间质谱技术分析分枝杆菌液体培养管污染菌菌群特征[J].新发传染病电子杂志,2024,9(2):7-10,4.基金项目
广西科技重大专项(桂科AA22096027) （桂科AA22096027）
