2型糖尿病患者血清miR-103a和miR-497水平表达与微血管病变发生的相关性研究OACSTPCD

Objective To explore the correlation between the expression of serum microRNA(miR)-103a and microRNA(miR)-497 and micro vascular disease(MVD)in patients with type 2 diabetes mellitus(T2DM).Methods Patients diagnosed with T2DM in Tangshan Union Medical College Hospital from May 2022 to April 2023 were selected as the study group(n=113),which were divided into type 2 diabetes microangiopathy(DMAP)group(n=51)and T2DM group(n=62)according to whether MVD was complicated.Healthy subjects in the same period were selected as control group(n=105).Clinical data were collected,and real-time fluorescence polymerase chain reaction(qRT-PCR)was applied to detect serum levels of miR-103a and miR-497.Logistic regression was applied to analyze the influencing factors of T2DM complicated with MVD.Receiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of serum miR-103a and miR-497 levels in T2DM complicated with MVD.Results The serum levels of miR-103a(0.62±0.13)and miR-497(0.79±0.14)in the study group were lower than those in the control group(0.96±0.16,1.03±0.18),with significant differences(t=17.273,11.031,all P＜0.05).The serum levels of miR-103a(0.53±0.08)and miR-497(0.69±0.10)in the DMAP group were lower than those in the T2DM group(0.69±0.10,0.87±0.13),with significant differences(t=9.247,8.108,all P＜0.05).The proportions of patients with a disease course of ≥ 8 years and concomitant hyperlipidemia,and the levels of FBG,TC,Scr and BUN in the DMAP group were higher than in the T2DM group,with significant differences(t/x2=8.294,15.342,-2.855,-5.659,-8.951,-3.880,all P＜0.05).The area under curve(AUC)of serum miR-103a,miR-497 and their combination in the diagnosis of T2DM with MVD were 0.899,0.897 and 0.970,respectively.The combined diagnosis effect was better than that of individual diagnosis(Z=2.268,2.267,all P＜0.05).The results of logistic regression analysis showed that FBG(OR=1.879,95％CI:1.262～2.797),TC(OR=2.141,95％CI:1.348～3.400),and Scr(OR=3.417,95％CI:1.569～7.440)were independent risk factors for T2DM with MVD(all P＜0.05),while miR-103a(OR=0.784,95％CI:0.648～0.948)and miR-497(OR=0.839,95％CI:0.750～0.938)were protective factors for T2DM with MVD(all P＜0.05).Conclusion The serum miR-103a and miR-497 were low in patients with T2DM complicated with MVD,which may be potential markers for the diagnosis of T2DM complicated with MVD.