中风与神经疾病杂志2024，Vol.41Issue(5)：420-425,6.DOI:10.19845/j.cnki.zfysjjbzz.2024.0081
巨噬细胞极化调控因子在脑卒中康复中的表达变化及其与认知功能恢复的关系
Changes in expression of macrophage polarization regulatory factor in stroke rehabilitation and its relationship with cognitive function recovery
摘要
Abstract
Objective To explore the changes in the expression of interferon regulatory factor 8(IRF8),a factor regulating macrophage polarization,in stroke rehabilitation and its relationship with cognitive function recovery.Methods This cross-sectional retrospective study included a cohort of patients with acute ischemic stroke treated in our hospital from January 2021 to January 2023.According to the Mini-Mental State Examination score two weeks after stroke,they were divided into post-stroke cognitive impairment(PSCI)group(n=76)and non-PSCI group(n=42).The expres-sion of the IRF8 gene in peripheral blood mononuclear cell(PBMC)was determined using RT-qPCR.The relationship be-tween IRF8 gene expression and cognitive function was studied by a binary logistic regression analysis.Results The ex-pression of IRF8 mRNA in PBMCs in the PSCI group was significantly higher than that in the non-PSCI group(P<0.001).There were significant differences in the proportions of PSCI and non-PSCI patients in the three groups by IRF8 mRNA ter-tiles[T1:53.8%(21/39),T2:64.1%(25/39),T3:75.0%(30/40),χ2=8.082,P=0.018].The logistic regression analy-sis showed that the IRF8 mRNA level>0.024(OR=1.956,P<0.001),age(OR=1.062,P<0.001),National Institutes of Health Stroke Scale(NIHSS)score(OR=1.145,P=0.014),and hemoglobin(OR=1.194,P=0.024)were significantly associated with a higher risk of PSCI.When the cut-off value was 0.01,the area under the curve(AUC)for IRF8 mRNA predicting PSCI was 0.682,with an accuracy of 71.49%,a specificity of 50.24%,and a sensitivity of 71.42%.Including IRF8 mRNA,age,NIHSS score,and hemoglobin into the nomogram,the AUC for PSCI prediction was 0.862.Conclusion The expression of IRF8 mRNA in PBMCs at admission is associated with the development of PSCI in the acute phase of stroke.关键词
巨噬细胞/列线图/脑卒中/干扰素调控因子8/认知功能Key words
Macrophage/Nomogram/Stroke/Interferon regulatory factor 8/Cognitive function分类
医药卫生引用本文复制引用
姚丽娜,戴胜燕,马艳,白康,张雯文,车雅男,纪利利,郭书琴..巨噬细胞极化调控因子在脑卒中康复中的表达变化及其与认知功能恢复的关系[J].中风与神经疾病杂志,2024,41(5):420-425,6.基金项目
河北省卫生健康委计划课题项目(2021WJ2033) （2021WJ2033）
