中国临床药理学杂志2024，Vol.40Issue(9)：1282-1286,5.DOI:10.13699/j.cnki.1001-6821.2024.09.009
大黄糖络丸对2型糖尿病大鼠肝损伤的影响及机制
Effects and mechanism of rhubarb Tangluo pill on liver injury in type 2 diabetic rats
摘要
Abstract
Objective To investigate the effect and mechanism of rhubarb Tangluo pill on liver injury in type 2 diabetic rats.Methods ZDF(fa/fa)rats were given high-fat diet to induce type 2 diabetes model,and were randomly divided into model group,positive control group(0.18 g·kg-1 metformin)and experimental-L,-M,-H groups(0.54,1.08 and 2.16 g·kg-1 rhubarb Tangluo pill),with 8 rats in each group.Eight ZDF(fa/+)rats were selected as control group.The control group and model group were given equal volume of pure water once a day for 12 weeks.An oral glucose tolerance test(OGTT)was performed after administration.Fasting blood glucose level,body mass and liver mass of rats were measured and liver index was calculated.The contents of glutamic-pyruvic transaminase(GPT),glutamic oxalacetic transaminase(GOT),triglyceride(TG)and total cholesterol(TC)in serum were detected.The histomorphologic changes of liver were observed by hematoxylin-eosin(HE)staining and Masson staining.The protein expression of phosphorylated insulin receptor substrate 1(p-IRS1),phosphorylated protein kinase B(p-Akt)and glucose transporter 4(GLUT4)were detected by Western blotting.Results After administration,the fasting blood glucose levels of control group,model group,positive control group and experimental-H group were(4.71±0.45),(29.9±2.97),(15.28±4.52)and(13.84±1.55)mmol·L-1,respectively;the liver index were 2.31±0.46,4.03±0.18,3.37±0.23 and 3.38±0.24;the relative expression level of p-IRS1 protein were 1.00±0.36,4.00±0.11,1.62±0.27 and 1.90±0.17,respectively;the relative expression levels of p-Akt protein were 1.00±0.25,0.21±0.04,0.73±0.15 and 0.54±0.04,respectively;GLUT4 protein relative expression levels were 1.00±0.11,0.40±0.08,0.86±0.04 and 0.70±0.06,respectively.Compared with the model group,the above indexes in the experimental-H group were statistically significant(P＜0.01,P＜0.05).Conclusion Rhubarb Tanglu pill can effectively improve glycolipid metabolism and liver injury in type 2 diabetes mellitus,and its mechanism may be related to the activation of IRS1/Akt signaling pathway.关键词
大黄糖络丸/2型糖尿病/肝损伤/胰岛素抵抗/胰岛素受体底物1/蛋白激酶B信号通路Key words
rhubarb Tangluo pills/type 2 diabetes/liver injury/insulin resistance/insulin receptor substrate 1/protein kinase B signaling pathway分类
医药卫生引用本文复制引用
安冬,梁永林,高艳奎,闫丰喆,白敏,赵思晨,刘中唐,聂宵..大黄糖络丸对2型糖尿病大鼠肝损伤的影响及机制[J].中国临床药理学杂志,2024,40(9):1282-1286,5.基金项目
甘肃省优秀博士生基金资助项目(23JRRA1218) （23JRRA1218）
甘肃省高等学校产业支撑计划基金资助项目(2021CYZC-03) （2021CYZC-03）
甘肃中医药大学研究生创新基金资助项目(2022CX05) （2022CX05）
