中国临床药理学杂志2024，Vol.40Issue(9)：1296-1300,5.DOI:10.13699/j.cnki.1001-6821.2024.09.012
基于Ⅱ型胶原诱导性关节炎模型大鼠探讨雷公藤多苷片的长期毒性研究
Study on long-term toxicity of Tripterygium glycosides tablets based on type Ⅱ collagen-induced arthritis model rats
摘要
Abstract
Objective To observe the multi-system toxicity of Tripterygium glycosides tablets in rats with type Ⅱ collagen-induced arthritis(CIA).Methods Healthy SD rats were randomly divided into normal group,model group and experimental group,with 10 rats in each group.In addition to the normal group,the other groups were established collagen-induced arthritis model.After the first immunization,the normal group and the model group were given an equal volume of 0.3％sodium carboxymethyl cellulose solution,and the experimental group was given 72 mg·kg-1·d-1 Tripterygium glycosides solution,once a day for 6 weeks.On the 42 nd day,the blood routine of each group was detected and the organ index was calculated.The levels of liver,kidney function and sex hormones in rats were detected by enzyme-linked immunosorbent assay.The histopathological changes of liver,kidney,ovary and testis were observed by hematoxylin-eosin(HE)staining.Results The testicular indexes of the normal group,the model group and the experimental group were(0.81±0.05)％,(0.97±0.06)％and(0.81±0.12)％;the estradiol contents were(63.90±16.19),(55.42±7.23)and(40.04±5.97)pg·mL-1;the testosterone contents were(1 290.96±257.94),(1 198.43±190.77)and(912.62±61.72)pg·mL-1,respectively.There were statistically significant differences in the above indexes between the model group and the experimental group(P＜0.01,P＜0.05).HE pathological results showed that Tripterygium glycosides tablets could cause abnormal histopathological changes of ovary and testis in CIA model rats.Conclusion Continuous administration of 8-fold clinical equivalent dose of Tripterygium glycosides tablets for 6 weeks can cause damage to the reproductive system of CIA rats.关键词
雷公藤多苷片/Ⅱ型胶原诱导性关节炎模型/肝肾毒性/血液系统毒性/生殖毒性Key words
Tripterygium glycosides tablet/type Ⅱ collagen-induced arthritis model/hepatorenal toxicity/hematological toxicity/reproductive toxicity分类
医药卫生引用本文复制引用
叶田香,石明珠,杨卫东,魏砚明,李会芳,程生辉..基于Ⅱ型胶原诱导性关节炎模型大鼠探讨雷公藤多苷片的长期毒性研究[J].中国临床药理学杂志,2024,40(9):1296-1300,5.基金项目
山西省基础研究计划基金资助项目(20210302123235) （20210302123235）
山西中医药大学科技创新团队基金资助项目(2022TD1016) （2022TD1016）
