中国临床药理学杂志2024，Vol.40Issue(9)：1301-1305,5.DOI:10.13699/j.cnki.1001-6821.2024.09.013
温阳化浊通络方对硬皮病小鼠肺微血管损伤的影响
Effect of Wenyang Huazhuo Tongluo recipe on pulmonary micro vascular injury in mice with scleroderma based on mitophagy
摘要
Abstract
Objective To explore the effect of Wenyang Huazhuo Tongluo recipe on pulmonary microvascular injury in mice with scleroderma based on mitophagy.Methods Fifty mice were randomly divided into blank control group(0.9％NaCl,by gavage),control group(0.9％NaCl,by gavage),model group,Wenyang Huazhuo Tongluo recipe group(47mg·kg-1·d-1 Wenyang Huazhuo Tongluo recipe by gavage),positive control group(10 mg·kg-1·d-1 KC7F2 dissolved in phosphate buffer solution intraperitoneal injection),continuous administration for 4 weeks.The expression levels of in vitro membrane translocation enzyme 20(TOMM20),hypoxia inducible factor-1α(HIF-1α),B cell lymphoma-2/adenovirus E1B-19 kDa interacting protein 3(BNIP3),PTEN inducible muscle enzyme protein 1(PINK1)and E3 ubiquitin ligase(Parkin)in lung tissue were detected by immunohistochemistry(IHC).Western blot(WB)was used to detect the expression levels of mitophagy-related proteins(TOMM20,LC3B)and HIF-1α/BNIP3/PINK1/Parkin pathway proteins in pulmonary microvascular endothelial cells.Results The relative content of HIF-1α in microvascular endothelial cells of lung tissue in the control group,model group,Wenyang Huazhuo Tongluo recipe group and positive control group were 0.17±0.02,0.98±0.01,0.66±0.03 and 0.48±0.01;the relative content of BNIP3 were 0.40±0.02,0.74±0.01,0.56±0.01 and 0.60±0.02;the relative content of PINK1 were 0.26±0.04,0.88±0.01,0.65±0.02 and 0.67±0.02;the relative contents of Parkin were 0.33±0.02,0.89±0.01,0.65±0.02 and 0.77±0.02;the relative contents of TOMM20 were 1.10±0.02,0.58±0.01,1.02±0.01 and 0.98±0.03;the relative contents of LC3B-Ⅰ/LC3B-Ⅱ were 0.24±0.01,0.80±0.01,0.53±0.02 and 0.70±0.02,respectively.The content of HIF-1α,BNIP3,PINK1,Parkin and LC3B-Ⅰ/LC3B-Ⅱ in model group was higher than those in control group.Wenyang Huazhuo Tongluo recipe can effectively reduce its content.The content of TOMM20 in the model group was lower than that in control group,and Wenyang Huazhuo Tongluo recipe can effectively increase its content.Conclusion Wenyang Huazhuo Tongluo recipe may inhibit mitophagy and improve SSc pulmonary microvascular injury by increasing TOMM20 and inhibiting the protein expression of LC3B and HIF-1α/BNIP3/PINK1/Parkin signaling pathway.关键词
温阳化浊通络方/硬皮病/线粒体自噬/缺氧诱导因子-1α/B细胞淋巴瘤-2/腺病毒E1B-19 kDa相互作用蛋白3/PTEN诱导性肌酶蛋白1/E3泛素连接酶Key words
Wenyang Huazhuo Tongluo recipe/scleroderma/mitochondrial autophagy/hypoxia inducible factor-1 alpha/B cell lymphoma-2/adenovirus E1B-19 kDa interacting protein 3/PTEN inducible muscle enzyme protein 1/E3 ubiquitin ligase分类
中医学引用本文复制引用
陈爽,李凯,卞博,郭克磊,卞华,刘畅,徐经纬..温阳化浊通络方对硬皮病小鼠肺微血管损伤的影响[J].中国临床药理学杂志,2024,40(9):1301-1305,5.基金项目
国家自然科学基金资助项目(81774300,82074415) （81774300,82074415）
