中国临床药理学杂志2024，Vol.40Issue(9)：1336-1340,5.DOI:10.13699/j.cnki.1001-6821.2024.09.020
用LC-MS/MS法同时测定人血浆中甘草酸及其活性代谢产物甘草次酸的浓度
Simutaneous determination of glycyrrhizin and its active metabolite glycyrrhetinic acid in human plasma by LC-MS/MS
黄辉 1邓远雄 2刘海艳 3吴慧慧3
作者信息
- 1. 湖南师范大学医学院,湖南长沙 410013||长沙晶易医药科技股份有限公司,湖南长沙 410205
- 2. 湖南师范大学医学院,湖南长沙 410013
- 3. 长沙晶易医药科技股份有限公司,湖南长沙 410205
- 折叠
摘要
Abstract
Objective To develop an LC-MS/MS method for the simutaneous determination of glycyrrhizin(GLY)and its main active metabolite glycyrrhetinic acid(GA)in human plasma.Methods Sample preparation was done by protein precipitation with methanol.Chromatographic separation was achieved by a Synergi TM 4 pm Fusion-RP 80A(2.0 mm × 50 mm,4 µm)column with a gradient mobile phase consisting of acetonitrile(containing 0.1％formic acid)-water(containing 0.1％formic acid),at a flow rate of 0.6 mL·min-1.GLY and GA were monitored using negative electrospray triple quadrupole mass spectrometer via multiple reaction monitoring(MRM)mode.The specificity,calibration curve and range,accuracy and precision,recovery,matrix effect,dilution integrity and stability were validated.Results The validated method had an excellent linearity in the range of 0.30-80.00 ng·mL-1 and 1.80-480.00 ng·mL-1 for GLY and GA,with the limits of quantification were 0.30 and 1.80 ng·mL-1.Recovery efficiencies were in the range of 98.14％-109.10％and 97.25％-100.87％for GLY and GA.No significant matrix effects were found for the two analytes.Intra-day and inter-day precisions of four quality control concentrations were less than 10.72％.Intra-day and inter-day accuracies were determined to be 95.38％-109.34％with all accuracy measurements.Conclusion The validated method was rapid,sensitive,selective and accurate for the quantification of GLY and its active metabolite GA,successfully used to the pharmacokinetics study of GLY and its active metabolite in human.关键词
甘草酸/甘草次酸/液相色谱串联质谱/药代动力学Key words
glycyrrhizin/glycyrrhetinic acid/liquid chromatography tandem mass spectrometry/pharmacokinetics分类
医药卫生引用本文复制引用
黄辉,邓远雄,刘海艳,吴慧慧..用LC-MS/MS法同时测定人血浆中甘草酸及其活性代谢产物甘草次酸的浓度[J].中国临床药理学杂志,2024,40(9):1336-1340,5.
