中国临床药理学杂志2024，Vol.40Issue(9)：1341-1344,4.DOI:10.13699/j.cnki.1001-6821.2024.09.021
超高效液相色谱-三重四极杆质谱法同时测定消肿膏中6种乌头型生物碱含量
Simultaneous determination of 6 aconitum-type alkaloids in Xiaozhong ointment by ultra-high performance liquid chromatography triple quadrupole mass spectrometry
摘要
Abstract
Objective To establish a method for the simultaneous determination of aconitine,neoaconitine,hypaconitine,benzoyl aconitine,benzoyl mesaconine and benzoylhypacoitine in Xiaozhong ointment by UPLC-TQD-MS.Methods ACQUITY UPLC BEH C18 column(50 mm ×2.1 mm,1.7 μm),mobile phase 0.1％formic acid water(A)-acetonitrile(B),gradient elution,column temperature 40 ℃,flow rate 0.3 mL·min-1,injection volume 5 μL;electrospray ionization source(ESI+)and multiple reaction monitoring(MRM)were used for mass spectrometry analysis.Results The concentration of aconitine,new aconitine,hypaconitine,benzoyl aconitine,benzoyl new aconitine and benzoyl hypaconitine were 1.0-100.0 ng·mL-1,respectively,the average recovery were 98.62％-101.24％.The mass fractions of the six components were 0.18,0.33,0.38,0.43,0.28,0.06μg·g-1.Conclusion The method can be used to determine the content of 6 aconitine-type alkaloids in Xiaozhong ointment,and provide reference for the quality evaluation and clinical safe use of Xiaozhong ointment.关键词
消肿膏/乌头型生物碱/超高效液相色谱-三重四极杆质谱法/含量测定Key words
Xiaozhong ointment/aconitine alkaloid/ultra-high performance liquid chromatography triple quadrupole mass spectrometry/determination分类
医药卫生引用本文复制引用
夏天利,赵德萍,薛傲,金美玲,朱紫悦,李卿,张宁,雷霞..超高效液相色谱-三重四极杆质谱法同时测定消肿膏中6种乌头型生物碱含量[J].中国临床药理学杂志,2024,40(9):1341-1344,4.基金项目
江苏省中医退行性骨关节病临床医学创新中心基金资助项目(苏中医科教[2021]4号) （苏中医科教[2021]4号）
