中国临床药理学杂志2024，Vol.40Issue(10)：1424-1428,5.DOI:10.13699/j.cnki.1001-6821.2024.10.007
基于PI3K/Akt/mTOR信号通路探讨归芪益元膏联合顺铂治疗Lewis肺癌的作用
Role of Guiqi Yiyuan ointment combined with cisplatin in the treatment of Lewis lung cancer based on PI3K/Akt/mTOR signal pathway
摘要
Abstract
Objective To observe the inhibitory effect of Guiqi Yiyuan ointment on tumor growth in mice with Lewis lung cancer,and to explore the molecular mechanism of Guiqi Yiyuan ointment combined with cisplatin through phosphoinositide 3-kinase/protein kinase B/mammalian rapamycin target protein(PI3K/Akt/mTOR)signal pathway.Methods Sixty C57BL/6 mice were randomly divided into 6 groups with 10 mice in each group.Except for the blank group(0.9％NaCl),Lewis lung cancer-bearing mice were randomly divided into model group(0.9％NaCl),control group(0.9％NaCl,cisplatin 5 mg·kg-1)and low,medium,high dose experimental groups(Guiqi Yiyuan ointment 1.6,3.3,6.6 g·kg-1,cisplatin 5 mg·kg-1).Flow cytometry was used to detect bone marrow-derived suppressor cells(MDSCs);the expression of related proteins in tumor tissues was detected by Western blot.Results The tumor inhibition rates in control group and low,medium,high dose experimental groups were(39.87±4.45)％,(45.74±14.97)％,(57.78±4.70)％and(69.82±11.05)％.The proportion of MDSCs in bone marrow of in blank group,model group,control group and low,medium,high dose experimental groups were(36.13±1.08)％,(68.63±2.94)％,(58.93±2.02)％,(58.00±1.50)％,(50.93±5.06)％and(43.07±2.41)％.The protein expressions of p-PI3K/PI3K in model group,control group and low,medium and high experimental groups were 0.97±0.03,0.77±0.02,0.72±0.01,0.68±0.03 and 0.53±0.02;PTEN were 0.21±0.07,0.65±0.07,0.74±0.06,0.99±0.13,1.11±0.13;p-Akt/Akt were 1.01±0.02,0.82±0.02,0.77±0.00,0.72±0.03 and 0.52±0.04;p-mTOR/mTOR were 1.01±0.01,0.76±0.05,0.69±0.07,0.59±0.06 and 0.47±0.06.There were significant differences between low,medium,high experimental groups and control group(all P＜0.05).Conclusion Guiqi Yiyuan ointment combined with cisplatin can significantly improve the quality of life and inhibit tumor growth in mice.The mechanism may be the inhibition of PI3K/Akt/mTOR signal pathway and the enhancement of tumor cell apoptosis and autophagy.关键词
归芪益元膏/顺铂/肺癌/磷脂酰化醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白信号通路/凋亡/自噬/增效减毒Key words
Guiqi Yiyuan ointment/cisplatin/lung cancer/phosphoinositide 3-kinase/protein kinase B/mammalian rapamycin target protein signal pathway/apoptosis/autophagy/synergism and detoxification分类
医药卫生引用本文复制引用
袁超,孔思琪,梁建庆,张毅,胡蓉,张悦,刘瑜,李金田..基于PI3K/Akt/mTOR信号通路探讨归芪益元膏联合顺铂治疗Lewis肺癌的作用[J].中国临床药理学杂志,2024,40(10):1424-1428,5.基金项目
国家自然科学基金资助项目(82160872) （82160872）
甘肃省教育厅"双一流"科研重点基金资助项目(GSSYLXM-05) （GSSYLXM-05）
甘肃省中医药研究中心开放课题基金资助项目(zyzx-2020-zx17) （zyzx-2020-zx17）
中医学一级学科"岐黄英才"导师专项基金资助项目(2022-13) （2022-13）
